In vitro: |
Agricultural and Biological Chemistry, 1983, 47(4):787-794. | Microbial Transformation of Manool and (12Z)-Labda- 8(17),12,14-triene by Rhodococcus erythropolis JTS-131.[Reference: WebLink] | Rhodococcus erythropolis JTS-131, a cis-abienol and sclareol transformable bacterium, converted manool and accumulated manoyl oxide in the culture broth.
METHODS AND RESULTS:
In the presence of metabolic inhibitors, 13β-hydroxylabda-8(17),14-dien-18-oic acid(13-Hydroxylabda-8(17),14-dien-18-oic acid) and its methylester (a new compound) were accumulated as the transformation products. This strain also transformed (12Z)-labda-8(17),12,14- triene and acqumulated (12Z)-labda-8(17),12,14-trien-18-al in the culture broth. In the presence of metabolic inhibitors, two compounds, (12Z)-labda-8(17),12,14-trien-18-ol and 4,18,19-trinor-3,4- seco-5-oxo-(12Z)-labda-8(17),12,14-trien-3-oic acid were accumulated.
CONCLUSIONS:
Based on the experiments on the degradation sequence, transformation pathways for these two labdanes by the bacterium are proposed. |
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