| Structure Identification: |
| Chinese Traditional & Herbal Drugs , 2016 ,47(12):2044-2052. | | Analysis on chemical constituents in Bupleuri Radix by HPLC-Q-TOF-MS[Reference: WebLink] | To investigate the chemical constituents in extract of Bupleuri Radix using HPLC-Q-TOF-MS.
METHODS AND RESULTS:
The HPLC separation achieved on Agilent 1200 HPLC Diamonsil Ⅱ C_(18) column(250 mm × 4.6 mm,5μm) was used with a mobile phase of0.05%H_2O-formic acid(A) and acetonitrile(B),the flow rate was 1.0 mL/min,the column temperature was 35℃,and the detection wavelength was 200-600 nm.Positive and negative ions MS information and element analysis,via comparing MS data with those of the standard compounds and coupled with the related literature were used to analyze the main chemical constituents of BupleuriRadix. Twenty-one chemical constituents were identified,including three phenolic acid,four flavonoids,and fourteen triterpenoid saponins,and isochlorogenic acid A(5),isochlorogenic acid B(6),7,3'-di-O-methylquercetin(8),and 5-Hydroxy-7-acetoxyflavone(24) were separated from Bupleuri Radix for the first time.
CONCLUSIONS:
It is used to quickly analyze the chemical constituents from Bupleuri Radix by establishing a rapid accurate evaluation method using HPLC-Q-TOF-MS,which could provide the references for the application development. |
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