| In vitro: |
| Chinese Journal of Pharmaceutical Analysis,2014,34(8):1391 -6. | | In vitro screening of potential xanthine oxidase inhibitors by high-performance liquid chromatography[Reference: WebLink] | To develop an HPLC method for In vitro screening of xanthine oxidase inhibitors.
METHODS AND RESULTS:
The activity of xanthine oxidase inhibitors was evaluated by the concentration of xanthine assayed by HPLC. The chromatographic conditions were as follows:column, Agilent SB-C18 (4.6 mm×250 mm, 5 μm); mobile phase, 0.02 mol·L-1 KH2PO4 (1% methanol); flow rate, 1.0 mL·min-1; detection wavelength, 254 nm; injection volume, 20 μL; column temperature, room temperature. Xanthine was perfectly separated from the other components under the selected chromatographic conditions. The xanthine oxidase inhibitory activity of 14 traditional Chinese medicines was evaluated by the above method. The 70% ethanol extracts of Quisqualis indica,Caesalpinia sappan,Polygonum cuspidatum and Paeonia suffruticosa showed significant activity with IC50 of 10. 04,8. 02,2. 06 and 1. 36 mg·mL- 1,respectively. Then,24 compounds isolated from C. sappan were evaluated by the above method. 7,3',4'-Trihydroxy-3-benzyl-2H-chromene,3-deoxysappanone B,sappanchalcone and protosappanin A showed xanthine oxidase inhibitory activity.
CONCLUSIONS:
This HPLC method is simple,accurate and suitable for in vitro screening of xanthine oxidase inhibitors. |
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