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Anatabine
Anatabine
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Anatabine
Price:
CAS No.: 581-49-7
Catalog No.: CFN96589
Molecular Formula: C10H12N2
Molecular Weight: 160.22 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Oil
Source: The seeds of tobacco (Nicotiana tabacum L.).
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Anatabine shows anti-inflammatory activity in vitro and in vivo, which is mediated in part via an inhibition of STAT3 phosphorylation.Anatabine has anti-Alzheimer's disease effects, it inhibits BACE-1 transcription and reduces BACE-1 protein levels in human neuronal like SHSY-5Y cells suggesting that the Aβ lowering properties of anatabine are mediated via a regulation of BACE-1 expression.
Targets: IL Receptor | Beta Amyloid | NF-kB | BACE | TNF-α | STAT | COX
In vitro:
Eur J Pharmacol. 2011 Nov 30;670(2-3):384-91.
Anatabine lowers Alzheimer's Aβ production in vitro and in vivo.[Pubmed: 21958873 ]
Brain Aβ accumulation represents a key pathological hallmark in Alzheimer's disease.
METHODS AND RESULTS:
In this study, we investigated the impact of Anatabine, a minor alkaloid present in plants of the Solanacea family on Aβ production in vitro using a cell line overexpressing the human amyloid precursor protein (APP) and in vivo using a transgenic mouse model of Alzheimer's disease. In vitro, Anatabine lowers Aβ₁₋₄₀ and Aβ₁₋₄₂ levels in a dose dependent manner and reduces sAPPβ production without impacting sAPPα levels suggesting that Anatabine lowers Aβ production by mainly impacting the β-cleavage of APP. Additionally, we show that Anatabine lowers NFκB activation at doses that inhibit Aβ production in vitro. Since NFκB is known to regulate BACE-1 expression (the rate limiting enzyme responsible for Aβ production), we determined the impact of Anatabine on BACE-1 transcription. We show that Anatabine inhibits BACE-1 transcription and reduces BACE-1 protein levels in human neuronal like SHSY-5Y cells suggesting that the Aβ lowering properties of Anatabine are mediated via a regulation of BACE-1 expression. In vivo, we show that an acute treatment with Anatabine for four days significantly lowers brain soluble Aβ₁₋₄₀ and Aβ₁₋₄₂ levels in a transgenic mouse model of Alzheimer's disease.
CONCLUSIONS:
Altogether our data suggest that Anatabine may represent an interesting compound for regulating brain Aβ accumulation.
In vivo:
Endocrinology. 2012 Sep;153(9):4580-7.
Anatabine ameliorates experimental autoimmune thyroiditis.[Pubmed: 22807490 ]
Tobacco smoking favorably influences the course of Hashimoto thyroiditis, possibly through the antiinflammatory proprieties of nicotine.
METHODS AND RESULTS:
In this study we tested Anatabine, another tobacco alkaloid, in a model of experimental autoimmune thyroiditis. Experimental autoimmune thyroiditis was induced by different doses of thyroglobulin, to produce a disease of low, moderate, or high severity, in 88 CBA/J female mice: 43 drank Anatabine supplemented water and 45 regular water. Mice were bled after immunization and killed to assess thyroid histopathology, thyroglobulin antibodies, T(4), and thyroid RNA expression of 84 inflammatory genes. We also stimulated in vitro a macrophage cell line with interferon-γ or lipopolysaccharide plus or minus Anatabine to quantitate inducible nitric oxide synthase and cyclooxygenase 2 protein expression. Anatabine reduced the incidence and severity of thyroiditis in the moderate disease category: only 13 of 21 mice (62%) developed thyroid infiltrates when drinking Anatabine as compared with 22 of 23 (96%) controls (relative risk 0.59, P = 0.0174). The median thyroiditis severity was 0.5 and 2.0 in Anatabine and controls, respectively (P = 0.0007 by Wilcoxon rank sum test). Anatabine also reduced the antibody response to thyroglobulin on d 14 (P = 0.029) and d 21 (P = 0.045) after immunization and improved the recovery of thyroid function on d 21 (P = 0.049). In the thyroid transcriptome, Anatabine restored expression of IL-18 and IL-1 receptor type 2 to preimmunization levels. Finally, Anatabine suppressed in a dose-dependent manner macrophage production of inducible nitric oxide synthase and cyclooxygenase 2.
CONCLUSIONS:
Anatabine ameliorates disease in a model of autoimmune thyroiditis, making the delineation of its mechanisms of action and potential clinical utility worthwhile.
Anatabine Description
Source: The seeds of tobacco (Nicotiana tabacum L.).
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
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Cell Metab. 2020 Mar 3;31(3):534-548.e5.
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Nature Plants. 2016 Dec 22;3: 16206.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 6.2414 mL 31.2071 mL 62.4142 mL 124.8284 mL 156.0355 mL
5 mM 1.2483 mL 6.2414 mL 12.4828 mL 24.9657 mL 31.2071 mL
10 mM 0.6241 mL 3.1207 mL 6.2414 mL 12.4828 mL 15.6035 mL
50 mM 0.1248 mL 0.6241 mL 1.2483 mL 2.4966 mL 3.1207 mL
100 mM 0.0624 mL 0.3121 mL 0.6241 mL 1.2483 mL 1.5604 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Eur J Pharmacol. 2013 Jan 5;698(1-3):145-53.
Anti-inflammatory activity of anatabine via inhibition of STAT3 phosphorylation.[Pubmed: 23178521 ]
Previous investigations have demonstrated the anti-inflammatory effects of cholinergic agonists, such as nicotine.
METHODS AND RESULTS:
In the present study, we investigated the potential anti-inflammatory activity of Anatabine, a minor tobacco alkaloid also present in plants of the Solanacea family which displays a chemical structural similarity with nicotine. Our data show that Anatabine prevents STAT3 and NFκB phosphorylation induced by lipopolysaccharide (LPS) or TNF-α in SH-SY5Y, HEK293, human microglia and human blood mononuclear cells. Using human whole blood, we found that Anatabine prevents IL-1β production induced by LPS. We assessed Anatabine's anti-inflammatory activity in vivo using an acute model of inflammation by challenging wild-type mice with LPS. We observed that Anatabine reduces pro-inflammatory cytokine production (IL-6, IL-1β and TNF-α) in the plasma, kidney and spleen of the animals following the injection of LPS and concomitantly opposes STAT3 phosphorylation induced by LPS in the spleen and kidney. We also investigated the impact of Anatabine on neuroinflammation using a transgenic mouse model of Alzheimer's disease (Tg APPsw) that displays elevated cytokine levels in the brain. Following a chronic oral treatment with Anatabine, a reduction in brain TNF-α and IL-6 levels compared to untreated Tg APPsw mice was observed. Moreover, an increased STAT3 phosphorylation was detected in the brains of Tg APPsw mice compared to wild-type littermates and was inhibited by Anatabine treatment.
CONCLUSIONS:
Overall our data show that the anti-inflammatory activity of Anatabine in vitro and in vivo is mediated in part via an inhibition of STAT3 phosphorylation.
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