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Bisline
Bisline
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Bisline
Price:
CAS No.: 30258-28-7
Catalog No.: CFN00350
Molecular Formula: C18H27NO6
Molecular Weight: 353.41 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Powder
Source: The herbs of Senecio othonniformis Fourcade.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison (Web)  (SDF)
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
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Biological Activity
Description: Reference standards.
In vitro:
J Anal Toxicol. 2004 Jan-Feb;28(1):11-5.
Simultaneous determination of isoline and its two major metabolites using high-performance liquid chromatography.[Pubmed: 14987418]

METHODS AND RESULTS:
A simple and reliable high-performance liquid chromatographic (HPLC) assay was developed for a simultaneous determination of isoline, a potent hepatotoxic pyrrolizidine alkaloid, and its two major metabolites, namely M1 (Bisline) and M2 (Bisline lactone, a new pyrrolizidine alkaloid). The latter two metabolites were produced during in vitro metabolism of isoline by rat and mouse microsomal enzyme systems. The analysis was conducted by a direct injection of aliquots of supernatant of the microsomal reaction mixture treated with the equal volume of ice-cold methanol onto a conventional reversed-phase analytical column (150 x 4.6 mm). The analytes were separated by a gradient elution with mobile phases A (0.01 M dihydro-potassium phosphate, pH 4.8) and B (acetonitrile). The assay has shown excellent precision and accuracy with less than 10% of overall intra- and interday variations and higher than 94% of overall accuracy.
CONCLUSIONS:
The developed HPLC method was successfully applied for the determination of the intact isoline and its two pH- and thermally labile metabolites produced in rat and mouse liver microsomal incubations.
Bisline Description
Source: The herbs of Senecio othonniformis Fourcade.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
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IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
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PMID: 32004475

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doi: 10.1016/j.molcel.2017.10.022.
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PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.8296 mL 14.1479 mL 28.2957 mL 56.5915 mL 70.7394 mL
5 mM 0.5659 mL 2.8296 mL 5.6591 mL 11.3183 mL 14.1479 mL
10 mM 0.283 mL 1.4148 mL 2.8296 mL 5.6591 mL 7.0739 mL
50 mM 0.0566 mL 0.283 mL 0.5659 mL 1.1318 mL 1.4148 mL
100 mM 0.0283 mL 0.1415 mL 0.283 mL 0.5659 mL 0.7074 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Phytochemistry. 2000 Aug;54(8):933-5.
Structure revision of isoline (ruwenine), bisline and isolinecic acid.[Pubmed: 11014292]

METHODS AND RESULTS:
X-ray crystallography of Bisline, and the chemical interconversion of Bisline and isoline (ruwenine), revealed that the structures previously assigned to these alkaloids required revision; as did that of isolinecic acid.
Journal of the Chemical Society C Organic,1970 ,17 (17) :2312-2315.
The Senecio alkaloids. Suggested structures for isoline and bisline, two new alkaloids from Senecio othonniformis Fourcade[Reference: WebLink]

METHODS AND RESULTS:
Plants of Senecio othonniformis Fourcade afforded the alkaloids, Bisline, C18H27NO6, and its acetate, isoline, C20H29NO7. Hydrolysis of isoline gave acetic acid, retronecine, and a new acid, isolinecic acid, C10H18O6. N.m.r. and mass spectral data and degradation studies indicate that isolinecic acid is 2,5-dihydroxy-3-methylheptane-2,5-dicarboxylic acid or its 2,4-dihydroxy-isomer.
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