| In vitro: |
| Virus Res. 2017 Jan 2;227:49-56. | | Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper.[Pubmed: 27686478 ] |
METHODS AND RESULTS:
A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), Bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica.
CONCLUSIONS:
All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40μM. | | J Nat Prod. 2007 Oct;70(10):1654-7. | | Screening of Indonesian medicinal plant extracts for antibabesial activity and isolation of new quassinoids from Brucea javanica.[Pubmed: 17896817] | Boiled extracts derived from 28 Indonesian medicinal plants were screened for their antibabesial activity against Babesia gibsoni in vitro.
METHODS AND RESULTS:
Of these extracts, the fruit of Brucea javanica was the most active in inhibiting parasite growth at a concentration of 10 microg/mL. Bioassay-guided fractionation of the fruit extract of Br. javanica led to the isolation of two new quassinoids, Bruceantinol B and bruceine J, and the structures of these compounds were elucidated on the basis of their spectroscopic data and by chemical transformation to known compounds. In addition, the known quassinoids bruceines A-D, Bruceantinol, and yadanziolide A were isolated.
CONCLUSIONS:
Antibabesial activities were also examined in vitro, and bruceine A and Bruceantinol were shown to be more potent than diminazene aceturate, a drug (IC50 = 103 ng/mL) used clinically against B. gibsoni, with IC50 values of 4 and 12 ng/mL, respectively. |
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