Science | Nature | Cell | View More
Natural Products
Cucurbitacin R
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Cucurbitacin R
Price:
CAS No.: 55903-92-9
Catalog No.: CFN92895
Molecular Formula: C30H46O7
Molecular Weight: 518.68 g/mol
Purity: >=98%
Type of Compound: Triterpenoids
Physical Desc.: Powder
Source: The rhizomes of Hemsleya amabilis.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Institute of Chinese Materia Me... (China)
  • Korea Intitute of Science and T... (Korea)
  • Universiti Malaysia Pahang (Malaysia)
  • Centralised Purchases Unit (CPU... (India)
  • University of Brasilia (Brazil)
  • Donald Danforth Plant Science C... (USA)
  • University of Oslo (Norway)
  • St. Jude Children Research Hosp... (USA)
  • University of Helsinki (Finland)
  • Johannes Gutenberg University M... (Germany)
  • University of Illinois at Chicago (USA)
  • More...
Package
Featured Products
Costunolide

Catalog No: CFN98928
CAS No: 553-21-9
Price: $40/20mg
Dehydropachymic acid

Catalog No: CFN92742
CAS No: 77012-31-8
Price: $268/10mg
Ganoderic acid C1

Catalog No: CFN92053
CAS No: 95311-97-0
Price: $218/5mg
Strictosamide

Catalog No: CFN98237
CAS No: 23141-25-5
Price: $158/10mg
Ginsenoside Rh4

Catalog No: CFN92594
CAS No: 174721-08-5
Price: $368/10mg
6'''-Feruloylspinosin

Catalog No: CFN90665
CAS No: 77690-92-7
Price: $138/20mg
Silydianin

Catalog No: CFN90242
CAS No: 29782-68-1
Price: $168/20mg
Glycitein

Catalog No: CFN99106
CAS No: 40957-83-3
Price: $70/20mg
Jionoside A1

Catalog No: CFN90809
CAS No: 120444-60-2
Price: $238/5mg
Vaccarin

Catalog No: CFN90131
CAS No: 53452-16-7
Price: $30/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Cucurbitacin R, a natural anti-inflammatory product, has been shown to exhibit activity against both adjuvant-induced arthritis and delayed-type hypersensitivity reactions induced by various agents; it has the potential to be a new immunosuppressive agent with antiproliferative effects through the inhibition of the NFAT with anti-inflammatory activity in DTH reactions. Cucurbitacin R can exert antitumorigenic activity in the absence of activated STAT3.
Targets: Bcl-2/Bax | Caspase | p21 | p53 | IL Receptor | NOS | PGE | TNF-α | COX | STAT
In vivo:
J Pharmacol Exp Ther. 2010 Feb;332(2):352-63.
Inhibition of delayed-type hypersensitivity by Cucurbitacin R through the curbing of lymphocyte proliferation and cytokine expression by means of nuclear factor AT translocation to the nucleus.[Pubmed: 19846588 ]
Cucurbitacin R is known to exhibit an anti-inflammatory effect in different experimental models of inflammation.
METHODS AND RESULTS:
In this article, we outline the effect of Cucurbitacin R on T lymphocyte proliferation, cytokine production, and nuclear factor activation, as well as its influence on various experimental models of delayed-type hypersensitivity (DTH) in mice. Cucurbitacin R reduced the proliferation of phytohemagglutinin A-stimulated human T lymphocytes (IC(50), 18 microM), modifying the cell cycle, as well as the production of cytokines [interleukin (IL)-2, IL-4, IL-10, and especially interferon-gamma] and the induction of the principal cyclins implicated in the cell cycle (A(1), B(1), D(2), and E). These effects are brought on by a novel, selective inhibition of nuclear factor AT (NFAT) by Cucurbitacin R, with no concomitant effect on other transcription factors such as activator protein-1. In addition, we tested the in vivo effects of Cucurbitacin R in three experimental models of DTH, as well as its effects on T lymphocyte proliferation, the cell cycle, cytokines, and cyclins. Although Cucurbitacin R was found to reduce the inflammatory response brought on by both oxazolone and dinitrofluorobenzene, its activity was even more pronounced against sheep red blood cell-induced edema in mouse paws, with a clear reduction in the production of IL-1beta, IL-4, and tumor necrosis factor alpha in the inflamed paw.
CONCLUSIONS:
In conclusion, Cucurbitacin R has the potential to be a new immunosuppressive agent with antiproliferative effects through the inhibition of the NFAT with anti-inflammatory activity in DTH reactions.
J Pharmacol Exp Ther. 2007 Feb;320(2):581-90.
Cucurbitacin R reduces the inflammation and bone damage associated with adjuvant arthritis in lewis rats by suppression of tumor necrosis factor-alpha in T lymphocytes and macrophages.[Pubmed: 17065367 ]
The aim of this study was to investigate the effects of Cucurbitacin R on an experimental model of adjuvant-induced arthritis in rats.
METHODS AND RESULTS:
The treatment of arthritic rats with Cucurbitacin R (1 mg/kg p.o. daily) modified the evolution of the clinical symptoms, whereas the histopathology of paws demonstrated a reduction in the signs of arthritis. Compared with the control group, radiography of the tibiotarsal joints of Cucurbitacin R-treated rats showed a decrease in joint damage and soft tissue swelling of the footpad. The in vivo study of the expression of proinflammatory enzymes (nitric-oxide synthase-2 and cyclooxygenase-2) with the aid of the Western blot technique, and that of tumor necrosis factor-alpha (TNF-alpha) and prostaglandin E(2) by means of enzyme-linked immunosorbent assays demonstrated a clear decrease in both the enzymes and the mediators in paw homogenates. The analysis for prostaglandin E(2), nitric oxide, and TNF-alpha production in RAW 264.7 macrophages, as well as that for TNF-alpha in human lymphocytes, indicated a reduction of all mediators. The expression of cyclooxygenase-2 was not modified in RAW 264.7 macrophages, whereas the expression of nitric-oxide synthase-2 was clearly diminished. Moreover, Cucurbitacin R was found to inhibit signal transducer and activator of transcription 3 activation in the lymphocytes of both healthy and arthritic men.
CONCLUSIONS:
These experimental data on the chronic model, together with previously reported activity on acute and subchronic experimental models, justify the anti-inflammatory activity of Cucurbitacin R and provide further evidence for the therapeutic potential of a group of natural products as anti-inflammatory agents.
Cucurbitacin R Description
Source: The rhizomes of Hemsleya amabilis.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Grosvenorin 2''-glucoside

Catalog No: CFN95282
CAS No: N/A
Price: $318/5mg
Cyclocurcumin

Catalog No: CFN95103
CAS No: 153127-42-5
Price: $338/10mg
Gynosaponin TN2

Catalog No: CFN95331
CAS No: 77658-95-8
Price: $318/5mg
Dihydrolicoisoflavone

Catalog No: CFN95073
CAS No: 164163-92-2
Price: $333/5mg
Verbasoside

Catalog No: CFN95015
CAS No: 61548-34-3
Price: $318/5mg
7-(4-Hydroxyphenyl)-1-phenyl-4-hep...

Catalog No: CFN95140
CAS No: 100667-52-5
Price: $318/5mg
Mahuannin B

Catalog No: CFN95554
CAS No: 82796-37-0
Price: $318/5mg
15-Deoxypulic acid

Catalog No: CFN95262
CAS No: 95523-05-0
Price: $368/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.928 mL 9.6399 mL 19.2797 mL 38.5594 mL 48.1993 mL
5 mM 0.3856 mL 1.928 mL 3.8559 mL 7.7119 mL 9.6399 mL
10 mM 0.1928 mL 0.964 mL 1.928 mL 3.8559 mL 4.8199 mL
50 mM 0.0386 mL 0.1928 mL 0.3856 mL 0.7712 mL 0.964 mL
100 mM 0.0193 mL 0.0964 mL 0.1928 mL 0.3856 mL 0.482 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Br J Pharmacol. 2010 Aug;160(7):1844-56.
Bcl-2 is a negative regulator of interleukin-1beta secretion in murine macrophages in pharmacological-induced apoptosis.[Pubmed: 20649584 ]
Cucurbitacin R, a natural anti-inflammatory product, has been shown to exhibit activity against both adjuvant-induced arthritis and delayed-type hypersensitivity reactions induced by various agents. Previous studies have demonstrated that the effects of Cucurbitacin R stem from its inhibition of both cytokine production and lymphocyte proliferation.
METHODS AND RESULTS:
Effects of Cucurbitacin R were investigated on lipopolysaccharide-stimulated RAW 264.7 cells. Cell cycle evolution was analysed by flow cytometry, detection of apoptosis by DNA ladder, Bcl-2, p21, p53, Bax, cleaved caspase-1 (p10), caspase-9, and caspase-3, cleaved caspase (p17) and interleukin-1beta detection was followed by Western blot analysis and mRNA expression with quantitative real time reverse transcription-polymerase chain reaction (qRT-PCR). Cucurbitacin R was found to induce apoptosis in lipopolysaccharide-stimulated RAW 264.7 macrophages through the inhibition of Bcl-2 expression, which regulates pro-inflammatory caspase-1 activation and interleukin-1beta release. Also, Cucurbitacin R arrested the cell cycle in the G(2)/M phase and increased the subG(0) population in lipopolysaccharide-stimulated RAW 264.7 macrophages. Moreover, it increased the expression of proteins p53 and p21, down-regulated the expression of Bcl-2, activated the activity of caspase-1 and augmented the production of interleukin-1beta. Finally, the transfection of RAW 264.7 macrophages with a Bcl-2 expression plasmid produced the inhibition of apoptosis and caspase-1 activation/interleukin-1beta release induced by Cucurbitacin R in RAW 264.7 cells.
CONCLUSIONS:
Taken together, these results point to a new apoptotic process in which interleukin-1beta release is directly regulated by Bcl-2 status; this contributes to the evidence that apoptotic processes do not induce inflammation.
Biochem Pharmacol. 2008 Jul 15;76(2):198-207.
Activated kRas protects colon cancer cells from cucurbitacin-induced apoptosis: the role of p53 and p21.[Pubmed: 18561895]
Cucurbitacins have been shown to inhibit proliferation in a variety of cancer cell lines. The aim of this study was to determine their biological activity in colon cancer cell lines that do not harbor activated STAT3, the key target of cucurbitacin. In order to establish the role of activated kRas in the responsiveness of cells to cucurbitacins, we performed experiments in isogenic colon cancer cell lines, HCT116 and Hke-3, which differ only by the presence of an activated kRas allele.
METHODS AND RESULTS:
We compared the activity of 23, 24-dihydrocucurbitacin B (DHCB) and Cucurbitacin R (CCR), two cucurbitacins that we recently isolated, with cucurbitacin I (CCI), a cucurbitacin with established antitumorigenic activity. We showed that cucurbitacins induced dramatic changes in the cytoskeleton (collapse of actin and bundling of tubulin microfilaments), inhibited proliferation and finally induced apoptosis of both HCT116 and Hke-3 cells. However, the presence of oncogenic kRas significantly decreased the sensitivity of cells to the three cucurbitacins tested, CCR, DHCB and CCI. We confirmed that mutational activation of kRas protects cells from cucurbitacin-induced apoptosis using nontransformed intestinal epithelial cells with inducible expression of kRasV12. Cucurbitacins induced the expression of p53 and p21 predominantly in HCT116 cells that harbor mutant Ras. Using HCT116 cells with targeted deletion of p53 or p21 we confirmed that p53 and p21 protect cells from apoptosis induced by cucurbitacins.
CONCLUSIONS:
These results demonstrated that sensitivity of human colon cancer cell lines to cucurbitacins depends on the kRas and p53/p21 status, and established that cucurbitacins can exert antitumorigenic activity in the absence of activated STAT3.
Costunolide

Catalog No: CFN98928
CAS No: 553-21-9
Price: $40/20mg
Dehydropachymic acid

Catalog No: CFN92742
CAS No: 77012-31-8
Price: $268/10mg
Ganoderic acid C1

Catalog No: CFN92053
CAS No: 95311-97-0
Price: $218/5mg
Strictosamide

Catalog No: CFN98237
CAS No: 23141-25-5
Price: $158/10mg
Ginsenoside Rh4

Catalog No: CFN92594
CAS No: 174721-08-5
Price: $368/10mg
6'''-Feruloylspinosin

Catalog No: CFN90665
CAS No: 77690-92-7
Price: $138/20mg
Silydianin

Catalog No: CFN90242
CAS No: 29782-68-1
Price: $168/20mg
Glycitein

Catalog No: CFN99106
CAS No: 40957-83-3
Price: $70/20mg
Jionoside A1

Catalog No: CFN90809
CAS No: 120444-60-2
Price: $238/5mg
Vaccarin

Catalog No: CFN90131
CAS No: 53452-16-7
Price: $30/20mg
Tags: buy Cucurbitacin R | Cucurbitacin R supplier | purchase Cucurbitacin R | Cucurbitacin R cost | Cucurbitacin R manufacturer | order Cucurbitacin R | Cucurbitacin R distributor