Structure Identification: |
J Chromatogr, 1971, 56(2):281-293. | Ion-exchange chromatography of soybean saponins1[Reference: WebLink] | METHODS AND RESULTS:
Resolution of soybean saponins on Dowex-1 is influenced by resin particle size, flow rate, temperature and eluting agent. Separations were good when columns of −400 mesh resin were operated at 50° and eluted at slow flow rates either acetic or propionic acid. A linear gradient of 1.5 to 3 N propionic acid separated the saponins into twelve fractions, many of which consisted of two or more components when analyzed by thin-layer chromatography. Glucuronic acid, glucose, galactose rhamnose, xylose and arabinose were found in acid hydrolyzates of all fractions, but relative amounts of the neutral sugars varied in most fractions.
CONCLUSIONS:
On analysis of the aglycones, three column fractions contained only soyasapogenol A, one contained only soyasapogenol E, while four consisted of soyasapogenol B plus small amounts of soyasapogenols C and D. Another fracion yielded a previously unreported aglycone which appears related to Soyasapogenol D. |
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