| Structure Identification: |
| Phytochemical Analysis, 2001, 12(4):255–262. | | HPLC‐UV and HPLC‐positive‐ESI‐MS analysis of the diterpenoid fraction from caper spurge (Euphorbia lathyris) seed oil.[Reference: WebLink] |
METHODS AND RESULTS:
Caper spurge (Euphorbia lathyris L.) seed oil contains a series of diterpenoids known as Euphorbia factors, or L‐factors, L1–L9(Euphorbia factor L1,Euphorbia factor L2, Euphorbia factor L3, Euphorbia factor L4 ,Euphorbia factor L5, Euphorbia factor L6, Euphorbia factor L7, Euphorbia factor L8, Euphorbia factor L9 ). They are esters of several polyols (lathyrol, epoxylathyrol, hydroxylathyrol and ingenol) and account for about 3–5% of the oil. The percentage of ingenol‐based L‐factors is very low, less than 5% of the diterpenoid fraction, but some of them (factors L5 and L6) are responsible for the irritant and co‐carcinogenic activities of the oil.
CONCLUSIONS:
This paper reports an HPLC‐UV and HPLC‐positive‐ESI‐MS analysis of the diterpenoid fraction of caper spurge seed oil before and after selective hydrolysis of ingenol‐based L‐factors. Separation of lathyrane polyols and esters, and ingenol and its esters was achieved using a chromatographic system consisting of a C18 stationary phase and acetonitrile:water as mobile phase. A new macrocyclic constituent, the deoxy Euphorbia factor L1, was identified in the oil. |
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