| Description: |
Licorisoflavan A shows weak scavenging activity against superoxide anion radical, it and licoricidin have potential for the development of novel host-modulating strategies for the treatment of cytokine and/or MMP-mediated disorders such as periodontitis.Licorisoflavan A has bactericidal effects on S. mutans UA159 at the concentration of ≥4 g/ml, it can be useful in developing oral hygiene products, such as gargling solutions and dentifrices for preventing dental caries. |
| Targets: |
p65 | NF-kB | AP-1 | MMP(e.g.TIMP) | IL Receptor |
| In vitro: |
| J Periodontol. 2011 Jan;82(1):122-8. | | Modulation of matrix metalloproteinase and cytokine production by licorice isolates licoricidin and licorisoflavan A: potential therapeutic approach for periodontitis.[Pubmed: 20722535 ] | Inflammatory cytokines and matrix metalloproteinases (MMPs) produced by resident and inflammatory cells in response to periodontopathogens play a major role in the tissue destruction observed in periodontitis, which is a disease that affects tooth-supporting structures. In the present study, we investigate the effects of licorice-derived licoricidin (LC) and Licorisoflavan A (LIA) on the secretion of various cytokines and MMPs by human monocyte-derived macrophages stimulated with Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) lipopolysaccharide (LPS).
METHODS AND RESULTS:
Macrophages were treated with non-toxic concentrations of LC or LIA before being stimulated with A. actinomycetemcomitans LPS. The secretion of cytokines and MMPs and the activation of nuclear factor-kappa B (NF-κB) p65 and activator protein (AP)-1 were assessed by enzyme-linked immunosorbent assays. LC and LIA inhibited the secretion of interleukin (IL)-6 and chemokine (C-C motif) ligand 5 in a concentration-dependent manner but did not affect the secretion of IL-8 by LPS-stimulated macrophages. LC and LIA also inhibited the secretion of MMP-7, -8, and -9 by macrophages. The suppression of cytokine and MMP secretion by LC and LIA was associated with the reduced activation of NF-κB p65 but not that of AP-1.
CONCLUSIONS:
The present study suggests that LC and LIA have potential for the development of novel host-modulating strategies for the treatment of cytokine and/or MMP-mediated disorders such as periodontitis. | | Caries Res. 2015;49(1):78-89. | | In vitro antimicrobial activities of 1-methoxyficifolinol, licorisoflavan A, and 6,8-diprenylgenistein against Streptococcus mutans.[Pubmed: 25531232 ] |
METHODS AND RESULTS:
Cell toxicity of substances to normal human gingival fibroblast (NHGF) cells was tested using a methyl thiazolyl tetrazolium assay. Chlorhexidine digluconate (CHX) was used in the control group. Three antimicrobial flavonoids, 1-methoxyficifolinol, Licorisoflavan A, and 6,8-diprenylgenistein, were isolated from the CLE. We found that the three flavonoids and CHX had bactericidal effects on S. mutans UA159 at the concentration of ≥4 and ≥1 μg/ml, respectively. The purified compounds completely inhibited biofilm development of S. mutans UA159 at concentrations over 4 μg/ml, which was equivalent to 2 μg/ml of CHX. Confocal analysis showed that biofilms were sparsely scattered in the presence of over 4 μg/ml of the purified compounds. However, the three compounds purified from CLE showed less cytotoxic effects on NHGF cells than CHX at these biofilm-inhibitory concentrations.
CONCLUSIONS:
Our results suggest that purified flavonoids from CLE can be useful in developing oral hygiene products, such as gargling solutions and dentifrices for preventing dental caries. |
|
| In vivo: |
| Fitoterapia. 2003 Dec;74(7-8):720-4. | | Antinephritis and radical scavenging activity of prenylflavonoids[Pubmed: 14630182] |
METHODS AND RESULTS:
Antinephritis activity of 5 prenylflavonoids similar to glabridin (1-5), isolated from Morus alba, Artocarpus communis, Glycyrrhiza uralensis and G. inflata, was evaluated in mice with glomerular disease (Masugi-nephritis). Oral administrations of artonin E (2) or licochalcone A (4) for 10 days (30 mg kg(-1) day(-1)) reduced the amount of urinary protein excretion compared to nephritic mice.
CONCLUSIONS:
ESR spectroscopy demonstrated that morusin (1) and Licorisoflavan A (5) increased the radical intensity of sodium ascorbate by about two times. Morusin, licoricidin (3), licochalcone A and Licorisoflavan A showed weak scavenging activity against superoxide anion radical. |
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