| Cell Research: |
| Tuberc Respir Dis (Seoul). 2014 Nov;77(5):203-8. | | Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells.[Pubmed: 25473407] | In this study, we investigated whether Lobetyolin, Lobetyol, and methyl linoleate derived from Codonopsis pilosula affect MUC5AC mucin secretion, production, and gene expression from airway epithelial cells.
METHODS AND RESULTS:
Confluent NCI-H292 cells were pretreated with Lobetyolin, Lobetyol, or methyl linoleate for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin gene expression, and mucin protein production and secretion were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.Lobetyolin, Lobetyol, and methyl linoleate inhibited the gene expression of MUC5AC mucin induced by PMA; Lobetyolin did not affect PMA-induced MUC5AC mucin production. However, Lobetyol and methyl linoleate inhibited the production of MUC5AC mucin; Lobetyolin and Lobetyol did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, methyl linoleate decreased the MUC5AC mucin secretion.
CONCLUSIONS:
These results suggest that among the three compounds, methyl linoleate can regulate gene expression, production, and secretion of MUC5AC mucin by directly acting on the airway epithelial cells. |
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