PD0325901(Mirdametinib) | CAS:391210-10-9

PD0325901(Mirdametinib)

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PD0325901(Mirdametinib) Description

Source:
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Kinase Assay:

Bioorg Med Chem Lett,2008 Dec 15;18(24):6501-4.

The discovery of the benzhydroxamate MEK inhibitors CI-1040 and PD 0325901.[Pubmed: 18952427]

Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of a glutathione S-transferase fusion protein containing p44MAP kinase (GST-MAPK) and a glutathione S-transferase protein containing p45MEK (GST-MEK). The assay solution contained 20 mM HEPES, pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 1 mM EGTA, 50 mM [gamma-32P]ATP, 10 mg GST-MEK, 0.5 mg GST-MAPK and 40 mg MBP in a final volume of 100 mL. Reactions are stopped after 20 minutes by addition of trichloroacetic acid and filtered through a GF/C filter mat. 32P retained on the filter mat is determined using a 1205 Betaplate. PD0325901 is assessed at various dose ranges in order to determine dose response curves.

Cell Research:

Mol Cancer Ther,2010 Jul;9(7):1968-76.

MEK inhibitor PD0325901 significantly reduces the growth of papillary thyroid carcinoma cells in vitro and in vivo.[Pubmed: 20587665]

Cell lines:PTC cells
Concentrations: 0.1 nM- 1 μM
Incubation Time: 48 hours
Method:
PTC cells (1 × 104) are seeded in 24-well plates with 1 mL of medium for 4 days in a 37 °C incubator. MEK inhibitor PD0325901 at varying concentrations is added to the cells in triplicate on day 0. MTT dissolved in 0.8% NaCl solution at 5 mg/mL is added to each well (0.2 mL) on day 2 to test GI50 or every day for cell growth curves. The cells are incubated at 37 °C for 3 hours with MTT. The liquid is then aspirated from the wells and discarded. Stained cells are dissolved in 0.5 mL of DMSO and their absorption at 570 nm is measured using a Synergy HT multidetection microplate reader. For GI50, cell growth is calculated as 100 × (T − T0)/(C − T0), where T is the optical density of the wells treated with inhibitors after a 48-hour period, T0 is the optical density at time zero, and C is the control optical density with DMSO only.

Animal Research:

Mol Cancer Ther,2010 Jul;9(7):1968-76.

MEK inhibitor PD0325901 significantly reduces the growth of papillary thyroid carcinoma cells in vitro and in vivo.[Pubmed: 20587665]

Animal Models: Ncr-nu/nu mice bearing PTC cells
Dosages: 20-25 mg/kg
Administration: Oral gavage

Product Name	PD0325901(Mirdametinib)
Price:
CAS No.:	391210-10-9
Catalog No.:	CFN60032
Molecular Formula:	C₁₆H₁₄F₃IN₂O₄
Molecular Weight:	482.19 g/mol
Purity:	>=98%
Type of Compound:	Alkaloids
Physical Desc.:	Powder
Source:
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS
Similar structural:	Comparison
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Alkaloids Compound Library MEK Inhibitor Library

Description:	Mirdametinib (PD0325901) is a selective and non ATP-competitive MEK inhibitor with IC50 of 0.33 nM in cell-free assays, roughly 500-fold more potent than CI-1040 on phosphorylation of ERK1 and ERK2.
Targets:	MEK \| ERK1/ERK2

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.0739 mL	10.3694 mL	20.7387 mL	41.4774 mL	51.8468 mL
5 mM	0.4148 mL	2.0739 mL	4.1477 mL	8.2955 mL	10.3694 mL
10 mM	0.2074 mL	1.0369 mL	2.0739 mL	4.1477 mL	5.1847 mL
50 mM	0.0415 mL	0.2074 mL	0.4148 mL	0.8295 mL	1.0369 mL
100 mM	0.0207 mL	0.1037 mL	0.2074 mL	0.4148 mL	0.5185 mL

CFN70477	7-Hydroxyflavonol	492-00-2	254.2	C₁₅H₁₀O₄
CFN93042	Glabrol	59870-65-4	392.49	C₂₅H₂₈O₄
CFN97828	Eicosyl ferulate	133882-79-8	474.73	C₃₀H₅₀O₄
CFN99637	Cimiside B	152685-91-1	752.9	C₄₀H₆₄O₁₃