Science | Nature | Cell | View More
Natural Products
Raddeanin A
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Raddeanin A
Price: $98 / 20mg
CAS No.: 89412-79-3
Catalog No.: CFN99985
Molecular Formula: C47H76O16
Molecular Weight: 897.11 g/mol
Purity: >=98%
Type of Compound: Triterpenoids
Physical Desc.: White powder
Source: The roots of Anemone raddeana Regel
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $24.2 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • University of Dicle (Turkey)
  • Universiti Kebangsaan Malaysia (Malaysia)
  • Almansora University (Egypt)
  • CSIRO - Agriculture Flagship (Australia)
  • Florida International University (USA)
  • Universidade de Franca (Brazil)
  • University of East Anglia (United Kingdom)
  • Vin?a Institute of Nuclear Scie... (Serbia)
  • Instituto Politécnico de Bragan?a (Portugal)
  • University of Wollongong (Australia)
  • Sapienza University of Rome (Italy)
  • More...
Package
Featured Products
Isochlorogenic acid B

Catalog No: CFN99119
CAS No: 14534-61-3
Price: $50/20mg
Pinocembrin

Catalog No: CFN98740
CAS No: 480-39-7
Price: $40/20mg
21-O-Tigloylgymnemagenin

Catalog No: CFN90978
CAS No: 1581276-63-2
Price: $268/5mg
(-)-Hinokinin

Catalog No: CFN92255
CAS No: 26543-89-5
Price: $ / mg
Paclitaxel

Catalog No: CFN97759
CAS No: 33069-62-4
Price: $30/20mg
N1,N10-Bis(p-coumaroyl)spermidine

Catalog No: CFN99248
CAS No: 114916-05-1
Price: $ /
Ginsenoside Rh3

Catalog No: CFN99972
CAS No: 105558-26-7
Price: $218/5mg
Allicin

Catalog No: CFN90201
CAS No: 539-86-6
Price: $50/20mg
Stylopine

Catalog No: CFN92812
CAS No: 84-39-9
Price: $ / mg
Isorhynchophylline

Catalog No: CFN96197
CAS No: 6859-01-4
Price: $138/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Raddeanin A, a histone deacetylases (HDACs) inhibitor, has high antiangiogenic potency, and antitumor activity, it can suppress the growth of liver and cells, it also inhibits proliferation of GC cells (BGC-823, SGC-7901 and MKN-28), induces their and inhibits the abilities of invasion, migration.
Targets: VEGFR | JAK | FAK | Src | Akt | HDAC | Bcl-2/Bax | Caspase | PARP | MMP(e.g.TIMP)
In vitro:
Phytomedicine. 2015 Jan 15;22(1):103-10.
Raddeanin A, a triterpenoid saponin isolated from Anemone raddeana, suppresses the angiogenesis and growth of human colorectal tumor by inhibiting VEGFR2 signaling.[Pubmed: 25636878]
Raddeanin A (RA) is an active triterpenoid saponin from a traditional Chinese medicinal herb, Anemone raddeana Regel. It was previously reported that RA possessed attractive antitumor activity through inhibiting proliferation and inducing apoptosis of multiple cancer cells. However, whether RA can inhibit angiogenesis, an essential step in cancer development, remains unknown.
METHODS AND RESULTS:
In this study, we found that RA could significantly inhibit human umbilical vein endothelial cell (HUVEC) proliferation, motility, migration, and tube formation. RA also dramatically reduced angiogenesis in chick embryo chorioallantoic membrane (CAM), restrained the trunk angiogenesis in zebrafish, and suppressed angiogenesis and growth of human HCT-15 colorectal cancer xenograft in mice. Western blot assay showed that RA suppressed VEGF-induced phosphorylation of VEGFR2 and its downstream protein kinases including PLCγ1, JAK2, FAK, Src, and Akt. Molecular docking simulation indicated that RA formed hydrogen bonds and hydrophobic interactions within the ATP binding pocket of VEGFR2 kinase domain.
CONCLUSIONS:
Our study firstly provides the evidence that RA has high antiangiogenic potency and explores its molecular basis, demonstrating that RA is a potential agent or lead candidate for antiangiogenic cancer therapy.
Biochem Biophys Res Commun. 2013 Sep 20;439(2):196-202.
Raddeanin A induces human gastric cancer cells apoptosis and inhibits their invasion in vitro.[Pubmed: 23988447]
Raddeanin A is one of the triterpenoid saponins in herbal medicine Anemone raddeana Regel which was reported to suppress the growth of liver and lung cancer cells. However, little was known about its effect on gastric cancer (GC) cells. This study aimed to investigate its inhibitory effect on three kinds of different differentiation stage GC cells (BGC-823, SGC-7901 and MKN-28) in vitro and the possible mechanisms.
METHODS AND RESULTS:
Proliferation assay and flow cytometry demonstrated Raddeanin A's dose-dependent inhibitory effect and determined its induction of cells apoptosis, respectively. Transwell assay, wounding heal assay and cell matrix adhesion assay showed that Raddeanin A significantly inhibited the abilities of the invasion, migration and adhesion of the BGC-823 cells. Moreover, quantitative real time PCR and Western blot analysis found that Raddeanin A increased Bax expression while reduced Bcl-2, Bcl-xL and Survivin expressions and significantly activated caspase-3, caspase-8, caspase-9 and poly-ADP ribose polymerase (PARP). Besides, Raddeanin A could also up-regulate the expression of reversion inducing cysteine rich protein with Kazal motifs (RECK), E-cadherin (E-cad) and down-regulate the expression of matrix metalloproteinases-2 (MMP-2), MMP-9, MMP-14 and Rhoc.
CONCLUSIONS:
In conclusion, Raddeanin A inhibits proliferation of human GC cells, induces their apoptosis and inhibits the abilities of invasion, migration and adhesion, exhibiting potential to become antitumor drug.
Raddeanin A Description
Source: The roots of Anemone raddeana Regel
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Nortrachelogenin-5'-C-beta-glucosi...

Catalog No: CFN95251
CAS No: 858127-39-6
Price: $318/10mg
Oxytroflavoside E

Catalog No: CFN95464
CAS No: 1391144-84-5
Price: $318/5mg
Gymnoside III

Catalog No: CFN95033
CAS No: 899430-03-6
Price: $238/10mg
3'-O-Methylviolanone

Catalog No: CFN95387
CAS No: 56973-42-3
Price: $318/5mg
Geissoschizine

Catalog No: CFN95566
CAS No: 439-66-7
Price: $413/5mg
7-(4-hydroxy-3-methoxyphenyl)-1-ph...

Catalog No: CFN95139
CAS No: 79559-60-7
Price: $268/20mg
Safflospermidine B

Catalog No: CFN95254
CAS No: N/A
Price: $413/5mg
Dehydrodicatechin A

Catalog No: CFN95451
CAS No: 36048-23-4
Price: $318/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.1147 mL 5.5735 mL 11.1469 mL 22.2938 mL 27.8673 mL
5 mM 0.2229 mL 1.1147 mL 2.2294 mL 4.4588 mL 5.5735 mL
10 mM 0.1115 mL 0.5573 mL 1.1147 mL 2.2294 mL 2.7867 mL
50 mM 0.0223 mL 0.1115 mL 0.2229 mL 0.4459 mL 0.5573 mL
100 mM 0.0111 mL 0.0557 mL 0.1115 mL 0.2229 mL 0.2787 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Chem Pharm Bull (Tokyo). 2014;62(8):779-85.
Synthesis and biological evaluation of Raddeanin A, a triterpene saponin isolated from Anemone raddeana.[Pubmed: 25087630]
First, Raddeanin A, a cytotoxic oleanane-type triterpenoid saponin isolated from Anemone raddeana REGEL, was synthesized. Stepwise glycosylation was adopted in the synthesis from oleanolic acid, employing arabinosyl, glucosyl and rhamnosyl trichloroacetimidate as donors.
METHODS AND RESULTS:
The chemical structure of Raddeanin A was confirmed by means of (1)H-NMR, (13)C-NMR, IR, MS and elemental analysis, which elucidated the structure to be 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranoside oleanolic acid.
CONCLUSIONS:
Biological activity tests showed that in the range of low concentrations, Raddeanin A displayed moderate inhibitory activity against histone deacetylases (HDACs), indicating that the HDACs' inhibitory activity of Raddeanin A may contribute to its cytotoxicity.
Structure Identification:
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Apr 1;923-924:43-7.
Determination of Raddeanin A in rat plasma by liquid chromatography-tandem mass spectrometry: application to a pharmacokinetic study.[Pubmed: 23455073]
A simple, rapid and sensitive LC-MS/MS analysis method was developed and validated for the determination of Raddeanin A (RA) in rat plasma. Protein precipitation with three volumes of methanol as the precipitation reagent was used as the sample preparation method.
METHODS AND RESULTS:
The analysis process was performed on a Thermo Syncronis C18 column with the mobile phase of methanol-water (containing 5mM ammonium formate, pH 2.2) (85:15, v/v). RA and glycyrrhetinic acid (internal standard) were monitored under negative electrospray ionization in multiple reaction monitoring (MRM) mode. Retention time of RA and IS were 2.1 min and 3.5 min, respectively. The limit of detection was 5 ng/mL and the linear range was 50-50,000 ng/mL. The intra-day and inter-day precision was 1.87-2.94% and 3.25-5.36%, and the intra-day and inter-day accuracy ranged from 5.9% to 10.5% and 5.6% to 11.1%, respectively. The absolute recovery was above 90.3%.
CONCLUSIONS:
The method has been successfully translated to the pharmacokinetic study of RA in rats after intravenous and intraperitoneal administration (0.75 mg/kg).
Isochlorogenic acid B

Catalog No: CFN99119
CAS No: 14534-61-3
Price: $50/20mg
Pinocembrin

Catalog No: CFN98740
CAS No: 480-39-7
Price: $40/20mg
21-O-Tigloylgymnemagenin

Catalog No: CFN90978
CAS No: 1581276-63-2
Price: $268/5mg
(-)-Hinokinin

Catalog No: CFN92255
CAS No: 26543-89-5
Price: $ / mg
Paclitaxel

Catalog No: CFN97759
CAS No: 33069-62-4
Price: $30/20mg
N1,N10-Bis(p-coumaroyl)spermidine

Catalog No: CFN99248
CAS No: 114916-05-1
Price: $ /
Ginsenoside Rh3

Catalog No: CFN99972
CAS No: 105558-26-7
Price: $218/5mg
Allicin

Catalog No: CFN90201
CAS No: 539-86-6
Price: $50/20mg
Stylopine

Catalog No: CFN92812
CAS No: 84-39-9
Price: $ / mg
Isorhynchophylline

Catalog No: CFN96197
CAS No: 6859-01-4
Price: $138/20mg
Tags: buy Raddeanin A | Raddeanin A supplier | purchase Raddeanin A | Raddeanin A cost | Raddeanin A manufacturer | order Raddeanin A | Raddeanin A distributor