| In vitro: |
| Food Chem Toxicol. 2012 Oct;50(10):3680-6. | | Lignan constituents of Tilia amurensis and their biological evaluation on antitumor and anti-inflammatory activities.[Pubmed: 22819933] | In the recent decade, numerous lignan derivatives isolated from plants have been proven to have the potential as an anti-cancer substance.
METHODS AND RESULTS:
On the search for anti-cancer compounds from Korean medicinal plants, the methanolic extract from the trunk of Tilia amurensis Rupr. (Tiliaceae) was found to have significant cytotoxicity against A549 (lung carcinoma), SK-OV-3 (ovary malignant ascites), SK-MEL-2 (skin melanoma), and HCT-15 (colon adenocarcinoma) in our screening test. Hence, a bioassay-guided fractionation and chemical investigation of the methanolic extract resulted in the isolation and identification of 10 lignan derivatives (1-10) including two new lignan glycosides named tiliamurosides A (1) and B (2). The structures of these new compounds were determined by spectroscopic methods, namely 1D and 2D nuclear magnetic resonance (NMR) techniques, high resolution mass spectrometry (HRMS), circular dichroism (CD) data, and chemical methods.
CONCLUSIONS:
Tiliamuroside B (2) and Schizandriside (3) showed significant cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines with inhibitory concentration (IC50) values of 3.26-8.89 μM. Moreover, (-)-syringaresinol (8) and (-)-pinoresinol 4-O-β-D-glucopyranoside (10) significantly inhibited nitric oxide (NO) production in murine microglia BV-2 with IC50 values of 15.05 and 34.35 μM, respectively. | | Arch Pharm Res. 2010 Dec;33(12):2011-6. | | Inhibitory effects of phenolic compounds from needles of Pinus densiflora on nitric oxide and PGE2 production.[Pubmed: 21191767 ] | The needles of Pinus densiflora Siebold et Zuccarini, a representative Pinus species that grows in Korea, have been used in oriental traditional medicine as remedies for rheumatitis, hemorrhage, cancer, etc.
METHODS AND RESULTS:
Phytochemical examination of the needles of Pinus densiflora Siebold et Zuccarini led to the isolation of four lignans, one flavan-3-ol, two flavonols and one organic acid. They were identified as icariside E(4) (1), cupressoside A (2), Schizandriside (3), (+)-isolariciresinol (4), (+)-catechin (5), quercetin 3-O-β-D-glucopyranoside (6), 5,7,8,4'-tetrahydroxy-3-methoxy-6-methylflavone 8-O-β-D-glucopyranoside (7) and (-)-shikimic acid (8).
CONCLUSIONS:
In order to evaluate the anti-inflammatory effects of these compounds, their inhibitory activities against nitric oxide and prostaglandin E(2) production in IFN-γ- and lipopolysaccharide-stimulated RAW 264.7 cells were examined. |
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Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)