| Structure Identification: |
| J Liq Chromatogr Relat Technol. 2014 Feb 1;37(4):572-587. | | Enrichment and purification of pedunculoside and syringin from the barks of Ilex rotunda with macroporous resins.[Pubmed: 25104900] | Jiubiying, the dried barks of Ilex rotunda Thunb. (Aquifoliaceae), has been used as herbal tea and traditional Chinese medicine for heat-clearing, detoxifying, dehumidification, and odynolysis.
METHODS AND RESULTS:
Pedunculoside and syringin are two main bioactive components. For the new drug development, we tried to isolate and purify several chemical constituents from Jiubiying by high-speed counter-current chromatography (HSCCC). The two-phase solvent system used was composed of ethyl acetate-n-butanol-water (1:6:7, v/v/v). From 1.0 g of Jiubiying extracts syringaresinol 4',4″-bis-O-β-D- glucopyranoside (I, 20.2 mg), syringin (II, 56.8 mg), Sinapaldehyde glucoside (III, 26.2 mg), syringaresinol 4'-O-β-D-glucopyranoside (IV, 20.4 mg), and pedunculoside (V, 45.1 mg) were obtained by one run of TBE-1000A HSCCC machine with 1000 mL of column volume. Their structures were identified by IR, MS, and extensive NMR studies.
CONCLUSIONS:
Syringaresinol 4',4″-bis-O-β-D-glucopyranoside (I) was isolated from this plant for the first time. |
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