| In vitro: |
| Phytother Res. 2010 Nov;24(11):1720-3. | | Anti-complement activity of isolated compounds from the roots of Clerodendrum bungei Steud.[Pubmed: 20648692] | To determine the anti-complement activity of natural diterpenes, chromatographic separation of the acetone-soluble fraction from the roots of Clerodendrum bungei (Verbenaceae) led to the isolation of five diterpenoids.
METHODS AND RESULTS:
An acetone-soluble extract of the roots of C. bungei exhibited significant anti-complement activity on the classical pathway complement system, which was expressed as total hemolytic activity. Five compounds isolated from the roots of C. bungei, namely 12-O-β-d-glucopyranosyl-3,11,16-trihydroxyabieta-8,11,13-triene (1), 3,12-O-β-d-diglucopyranosyl-11,16-dihydroxyabieta-8,11,13-triene (2), ajugaside A (3), Uncinatone (4) and 19-hydroxyteuvincenone F (5). Compounds 1, 2, 3, 4 and 5 showed inhibitory activity against complement system with 50% inhibitory concentrations (IC(50)) values of 24 µm, 138 µm, 116 µm, 87 µm and 232 µm.
CONCLUSIONS:
Among the compounds tested, 1 showed the most potent anti-complement activity (IC(50), 24 µm). | | Nat Prod Res. 2016 Feb 2:1-6. | | A new bioactive diterpenoid from pestalotiopsis adusta, an endophytic fungus from clerodendrum canescens.[Pubmed: 26831947] |
METHODS AND RESULTS:
Bioassay-guided fractionation of the culture extract of Pestalotiopsis adusta, an endophytic fungus isolated from the medicinal plant Clerodendrum canescens, led to the isolation of one new, (10S)-12,16-epoxy-17(15→16)-abeo-3,5,8,12,15-abietapentaen-2,7,11,14-tetraone (1), and four known diterpenoids, teuvincenone F (2), Uncinatone (3), coleon U (4), coleon U-12-methyl ether (5). These structures were identified by using spectroscopic methods, including UV, MS, 1D and 2D NMR experiments.
CONCLUSIONS:
This is the first report of these compounds being isolated from a Pestalotiopsis species. The cytotoxic activities of the compounds were evaluated, and compounds 1 and 3 demonstrated cytotoxic activities against the HL-60 tumour cell line (IC50 ＜ 20 μM). |
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