Structure Identification: |
Bioorganic & medicinal chemistry, 2001, 9(6):1499-1507. | Structures of withanosides I, II, III, IV, V, VI, and VII, new withanolide glycosides, from the roots of Indian Withania somnifera Dunal. and inhibitory activity for tachyphylaxis to clonidine in isolated guinea-pig ileum.[Reference: WebLink] | METHODS AND RESULTS: Seven new withanolide glycosides called withanoside I, withanoside II, withanoside III, withanoside IV, Withanoside V, Withanoside VI, and Withanoside VII were isolated from an Indian natural medicine, Ashwagandha, the roots of Indian Withania somnifera Dunal. (Solanaceae), together with four known compounds, withaferin A, 5α,20αF(R)-dihydroxy-6α,7α-epoxy-1-oxowitha-2,24-dienolide, physagulin D, and coagulin Q. The structures of withanoside I, withanoside II, withanoside III, withanoside IV, Withanoside V, Withanoside VI, and Withanoside VII were determined based on chemical and physicochemical evidence.
CONCLUSIONS:
Principal constituents, Withanoside VI (10 and 30 μM) and withaferin A (10 μM), attenuated the tachyphylaxis to clonidine on electrically stimulated guinea-pig ileum in vitro.
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