7-Amino-4-methylcoumarin | CAS:106562-32-7

7-Amino-4-methylcoumarin

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Description:

7-Amino-4-methylcoumarin is more suitable as a substrate for enteropeptidase than GD(4)K-NA.

In vitro:

BMB Rep. 2011 Jul;44(7):458-61.

A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin.[Pubmed: 21777516]

Enteropeptidase is a serine protease secreted by the pancreas and converts inactive trypsinogen to active trypsin. Enteropeptidase cleaves the C-terminal end of the substrate recognition sequence Asp-Asp-Asp-Asp-Lys (D(4)K). The assay for enteropeptidase has utilized GD(4)K-conjugated 2-naphthylamine (GD(4)K-NA) as a fluorogenic probe over the last 30 years. However, no other D(4)K-conjugated fluorogenic substrates of enteropeptidase have been reported. Furthermore, naphthalene is known as carcinogenic to humans.
METHODS AND RESULTS:
In this study, we used shift in the emission spectrum of GD(4)K-conjugated 7-Amino-4-methylcoumarin (GD(4)K-AMC) as a fluorogenic method to measure enteropeptidase activity. The kinetic analysis revealed that enteropeptidase has a K(M) of 0.025 mM and a k(cat) of 65 sec(-1) for 7-Amino-4-methylcoumarin, whereas it has a K(M) of 0.5 to 0.6 mM and a k(cat) of 25 sec(-1) for GD(4)K-NA. The optimum pH of 7-Amino-4-methylcoumarin hydrolysis was pH 8.0.
CONCLUSIONS:
Our data indicate that 7-Amino-4-methylcoumarin is more suitable as a substrate for enteropeptidase than GD(4)K-NA.

7-Amino-4-methylcoumarin Description

Source:	The herbs of Guazuma ulmifolia
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Product Name	7-Amino-4-methylcoumarin
Price:
CAS No.:	106562-32-7
Catalog No.:	CFN90420
Molecular Formula:	C₁₂H₁₁NO₄
Molecular Weight:	233.22 g/mol
Purity:	>=98%
Type of Compound:	Coumarins
Physical Desc.:	Cryst.
Source:	The herbs of Guazuma ulmifolia
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS SDF
Similar structural:	Comparison (Web) (SDF)
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Coumarins Compound Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	4.2878 mL	21.439 mL	42.878 mL	85.7559 mL	107.1949 mL
5 mM	0.8576 mL	4.2878 mL	8.5756 mL	17.1512 mL	21.439 mL
10 mM	0.4288 mL	2.1439 mL	4.2878 mL	8.5756 mL	10.7195 mL
50 mM	0.0858 mL	0.4288 mL	0.8576 mL	1.7151 mL	2.1439 mL
100 mM	0.0429 mL	0.2144 mL	0.4288 mL	0.8576 mL	1.0719 mL

CFN97429	7-Hydroxy-3-prenylcoumarin	86654-26-4	230.3	C₁₄H₁₄O₃
CFN98374	7-Hydroxy-6-methoxy-3-prenylcoumarin	299159-90-3	260.3	C₁₅H₁₆O₄
CFN89287	3-(1,1-Dimethylallyl)-8-hydroxy-7-methoxycoumarin	61899-42-1	260.28	C₁₅H₁₆O₄
CFN89033	Chalepensin	13164-03-9	254.28	C₁₆H₁₄O₃
CFN89223	Rutamarin	14882-94-1	356.41	C₂₁H₂₄O₅
CFN89021	Evodosin A	1291053-38-7	280.27	C₁₄H₁₆O₆
CFN97812	12-Oxocalanolide A	161753-49-7	368.43	C₂₂H₂₄O₅
CFN99466	Calanolide E	142566-61-8	388.5	C₂₂H₂₈O₆
CFN90420	7-Amino-4-methylcoumarin	106562-32-7	233.22	C₁₂H₁₁NO₄