Afatinib (BIBW2992) | CAS:439081-18-2 | Manufacturer ChemFaces

Afatinib (BIBW2992)

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Size /Price /Stock	10 mM * 1 mL in DMSO / Inquiry
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Afatinib (BIBW2992) Description

Source:
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Cell Research:

Oncogene,2008 Aug 7;27(34):4702-11.

BIBW2992, an irreversible EGFR/HER2 inhibitor highly effective in preclinical lung cancer models.[Pubmed: 18408761]

Cell lines:NSCLC cells
Concentrations: 0-10 μM
Incubation Time: 1 hour
Method:
1 × 104 NSCLC cells are transferred into each well of a 96-well plate and cultured overnight in serum-free media for the EGFR phosphorylation assay. After addition of BIBW2992 on the next day, the plates are incubated at 37 °C for 1 hour. EGF-stimulation is done using 100 ng/mL for 10 min at room temperature. Cells are washed with ice cold PBS, extracted with 120 μL HEPEX buffer per well and shaken at room temperature for 1 hour. In all 2 × 104 cells per well is used for the HER2 phosphorylation assay. Streptavidin precoated plates are coated with anti-EGFR-biotin at 1:100 dilution in blocking buffer and c-erb2/HER2 oncoprotein Ab-5(Clone N24)-Biotin. Cell extracts is then transferred to the antibody-coated wells and incubated at room temperature for 1 hour. Extinction is measured at 450 nm.

Animal Research:

Oncogene,2008 Aug 7;27(34):4702-11.

BIBW2992, an irreversible EGFR/HER2 inhibitor highly effective in preclinical lung cancer models.[Pubmed: 18408761]

Animal Models: Athymic NMRI-nu/nu female mice
Dosages: 20 mg/kg
Administration: Oral administration

Product Name	Afatinib (BIBW2992)
Price:
CAS No.:	439081-18-2
Catalog No.:	CFN60034
Molecular Formula:	C₂₄H₂₅ClFN₅O₃
Molecular Weight:	485.94 g/mol
Purity:	>=98%
Type of Compound:	Alkaloids
Physical Desc.:	Powder
Source:
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS
Similar structural:	Comparison
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Alkaloids Compound Library Autophagy Inhibitor Library

Description:	Afatinib (BIBW2992) inhibits EGFR/ErbB irreversibly in vitro with IC50 of 0.5, 0.4, 10, 14, 1 nM for EGFRwt, EGFR L858R , EGFR L858R/T790M ErbB2 (HER2) and ErbB4 (HER4), respectively. Afatinib induces autophagy.
Targets:	EGFR/ErbB \| Autophagy

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.0579 mL	10.2893 mL	20.5787 mL	41.1573 mL	51.4467 mL
5 mM	0.4116 mL	2.0579 mL	4.1157 mL	8.2315 mL	10.2893 mL
10 mM	0.2058 mL	1.0289 mL	2.0579 mL	4.1157 mL	5.1447 mL
50 mM	0.0412 mL	0.2058 mL	0.4116 mL	0.8231 mL	1.0289 mL
100 mM	0.0206 mL	0.1029 mL	0.2058 mL	0.4116 mL	0.5145 mL

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