| J Antibiot (Tokyo). 2001 Aug;54(8):650-7. |
| Effects of aflastatin A, an inhibitor of aflatoxin production, on aflatoxin biosynthetic pathway and glucose metabolism in Aspergillus parasiticus.[Pubmed: 11592501] |
Aflastatin A inhibits aflatoxin production by Aspergillus parasiticus via an unknown mechanism.
METHODS AND RESULTS:
We found that Aflastatin A clearly inhibited production of norsolorinic acid, an early biosynthetic intermediate of aflatoxin, at a concentration of 0.25 microg/ml. Reverse-transcriptase polymerase chain reaction (RT-PCR), and real-time quantitative PCR (TaqMan PCR) experiments indicated that the transcription of genes encoding aflatoxin biosynthetic enzymes (pksA, ver-1, and omtA) and a gene encoding a regulatory protein for expression of the biosynthetic enzymes (aflR) were significantly reduced by the addition of Aflastatin A. We also found that Aflastatin A elevated the glucose consumption and ethanol accumulation by A. parasiticus, and repressed transcription of genes involved in ethanol utilization.
CONCLUSIONS:
These results suggest that Aflastatin A inhibits a very early step in aflatoxin biosynthesis prior to the transcription of aflR and can influence glucose metabolism in the fungus. |
| J Antibiot (Tokyo). 1997 Feb;50(2):111-8. |
| Aflastatin A, a novel inhibitor of aflatoxin production by aflatoxigenic fungi.[Pubmed: 9099219] |
Aflastatin A, a novel inhibitor of the production of aflatoxin by aflatoxigenic fungi, has been isolated from the solvent extract of mycelial cake of Streptomyces sp. and its molecular formula was determined as C62H115NO24.
METHODS AND RESULTS:
Aflastatin A completely inhibited aflatoxin production by Aspergillus parasiticus NRRL 2999 in liquid medium or on agar plate at a concentration of 0.5 microgram/ml. The mycelial growth of this fungus was not affected in the liquid medium at the same concentration, while the hyphal extension rate was reduced on the plate together with some morphological changes. The growth of the fungus was not completely inhibited even at a concentration of 100 micrograms/ml.
CONCLUSIONS:
Aflastatin A exhibits antimicrobial activity against some bacteria, yeasts and fungi as well as antitumor activity. |
| Microbiology. 2001 Sep;147(Pt 9):2623-8. |
| Inhibitory effect of aflastatin A on melanin biosynthesis by Colletotrichum lagenarium.[Pubmed: 11535802] |
Aflastatin A, a novel inhibitor of the production of aflatoxin by aflatoxigenic fungi, has been isolated from the solvent extract of mycelial cake of Streptomyces sp. and its molecular formula was determined as C62H115NO24.
METHODS AND RESULTS:
Aflastatin A completely inhibited aflatoxin production by Aspergillus parasiticus NRRL 2999 in liquid medium or on agar plate at a concentration of 0.5 microgram/ml. The mycelial growth of this fungus was not affected in the liquid medium at the same concentration, while the hyphal extension rate was reduced on the plate together with some morphological changes. The growth of the fungus was not completely inhibited even at a concentration of 100 micrograms/ml.
CONCLUSIONS:
Aflastatin A exhibits antimicrobial activity against some bacteria, yeasts and fungi as well as antitumor activity. |
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