Broussoflavonol B | CAS:99217-70-6 | Manufacturer ChemFaces

Broussoflavonol B

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Description:

Broussoflavonol B is a potent growth inhibitor of ER-negative breast cancer stem-like cells, may be used to treat breast cancer. Broussoflavonol B increases the growth inhibitory activity of tamoxifen in tumorsphere cells derived from MCF7/TAM cells.

Targets:

Estrogen receptor

In vitro:

Eur J Pharmacol. 2013 Aug 15;714(1-3):56-64.

A novel anticancer agent Broussoflavonol B downregulates estrogen receptor (ER)-α36 expression and inhibits growth of ER-negative breast cancer MDA-MB-231 cells.[Pubmed: 23769740]

Estrogen receptor (ER)-negative breast cancers are aggressive and unresponsive to antiestrogens, and current therapeutic modalities for ER-negative breast cancer patients are usually associated with strong toxicity and side effects. Less toxic and more effective targeted therapies are urgently needed to treat this type of breast cancer.
METHODS AND RESULTS:
Here, we report that Broussoflavonol B, a chemical purified from the bark of the Paper Mulberry tree (Broussonetia papyrifera) exhibited potent growth inhibitory activity in ER-negative breast cancer MDA-MB-231 cells at sub-micromolar concentrations. Broussoflavonol B induced cell cycle arrest at both the G₀/G₁ and G₂/M phases accompanied by a downregulation of c-Myc protein, a upregulation of the cell cycle inhibitory proteins p16(INK4a), p19(INK4D) and p21(WAF1/CIP1) and a down-regulation of the expression levels of the G₂/M regulatory proteins such as cyclin B1, cdc2 and cdc25C. Broussoflavonol B also induced apoptotic cell death characterized by accumulation of the annexin V- and propidium iodide-positive cells, and cleavage of caspases 8, 9 and 3. In addition, Broussoflavonol B treatment also decreased the steady state levels of the epidermal growth factor receptor (EGFR) and ER-α36, a variant of estrogen receptor-α, and restricted growth of the stem-like cells in ER-negative breast cancer MDA-MB-231 cells.
CONCLUSIONS:
Our results thus indicate that Broussoflavonol B is a potent growth inhibitor for ER-negative breast cancer cells and provide a rational for preclinical and clinical evaluation of Broussoflavonol B for ER-negative breast cancer therapy.

Broussoflavonol B Description

Source:	The herbs of Broussonetia papyrifera
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Kinase Assay:

Am J Cancer Res. 2015 Jan 15;5(2):530-44.

Downregulation of ER-α36 expression sensitizes HER2 overexpressing breast cancer cells to tamoxifen.[Pubmed: 25973295]

Tamoxifen provided a successful treatment for ER-positive breast cancer for many years. However, HER2 overexpressing breast cancer cells respond poorly to tamoxifen therapy presumably by pass. The molecular mechanisms underlying development of tamoxifen resistance have not been well established. Recently, we reported that breast cancer cells with high levels of ER-α36, a variant of ER-α, were resistant to tamoxifen and knockdown of ER-α36 expression in tamoxifen resistant cells with the shRNA method restored tamoxifen sensitivity, indicating that gained ER-α36 expression is one of the underlying mechanisms of tamoxifen resistance.
METHODS AND RESULTS:
Here, we found that tamoxifen induced expression of ER-α36-EGFR/HER2 positive regulatory loops and tamoxifen resistant MCF7 cells (MCF7/TAM) expressed enhanced levels of the loops. Disruption of the ER-α36-EGFR/HER2 positive regulatory loops with the dual tyrosine kinase inhibitor Lapatinib or ER-α36 down-regulator Broussoflavonol B in tamoxifen resistant MCF7 cells restored tamoxifen sensitivity. In addition, we also found both Lapatinib and Broussoflavonol B increased the growth inhibitory activity of tamoxifen in tumorsphere cells derived from MCF7/TAM cells. Our results thus demonstrated that elevated expression of the ER-α36-EGFR/HER2 loops is one of the mechanisms by which ER-positive breast cancer cells escape tamoxifen therapy.
CONCLUSIONS:
Our results thus provided a rational to develop novel therapeutic approaches for tamoxifen resistant patients by targeting the ER-α36-EGFR/HER2 loops.

Product Name	Broussoflavonol B
Price:
CAS No.:	99217-70-6
Catalog No.:	CFN92774
Molecular Formula:	C₂₆H₂₈O₇
Molecular Weight:	452.5 g/mol
Purity:	>=98%
Type of Compound:	Flavonoids
Physical Desc.:	Yellow powder
Source:	The herbs of Broussonetia papyrifera
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS SDF
Similar structural:	Comparison (Web) (SDF)
Guestbook:

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Anticancer Compound Library Flavonoids Compound Library Estrogen receptor Inhibitor Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.2099 mL	11.0497 mL	22.0994 mL	44.1989 mL	55.2486 mL
5 mM	0.442 mL	2.2099 mL	4.4199 mL	8.8398 mL	11.0497 mL
10 mM	0.221 mL	1.105 mL	2.2099 mL	4.4199 mL	5.5249 mL
50 mM	0.0442 mL	0.221 mL	0.442 mL	0.884 mL	1.105 mL
100 mM	0.0221 mL	0.1105 mL	0.221 mL	0.442 mL	0.5525 mL

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CFN91888	Citrusinol	112516-43-5	352.34	C₂₀H₁₆O₆
CFN92774	Broussoflavonol B	99217-70-6	452.5	C₂₆H₂₈O₇
CFN96053	8-Lavandulylkaempferol	883859-83-4	422.5	C₂₅H₂₆O₆
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CFN91905	Cudraflavone B	19275-49-1	420.45	C₂₅H₂₄O₆
CFN98528	Noricaritin	5240-95-9	372.37	C₂₀H₂₀O₇
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