| Cell Research: |
| Int J Radiat Biol. 2015 May;91(5):399-406. | | Hypocrellin B in hepatocellular carcinoma cells: Subcellular localization and sonodynamic damage.[Pubmed: 25565557] | To study subcellular localization of Hypocrellin B in hepatocellular carcinoma cells, and Hypocrellin B-mediated sonodynamic action-induced cell damage.
METHODS AND RESULTS:
After incubation with 2.5 μM of Hypocrellin B, human hepatocellular carcinoma HepG2 cells were exposed to ultrasound waves for 8 sec at an intensity of 0.46 W/cm(2). Additionally, subcellular localization of Hypocrellin B in HepG2 cells with organelle probe staining was also observed using CLSM. R. The colony forming units of HepG2 cells decreased substantially after sonodynamic treatment. The results of TEM showed microvilli disappearance, apoptotic body formation, swollen mitochondria with loss of cristae and mitochondrial myelin-like features (or membrane whorls). Collapse of MMP was found in the treated cells. Hypocrellin B was distributed in mitochondria and lysosomes as well as in endoplasmic reticulum and Golgi apparatus.
CONCLUSIONS:
The findings demonstrated that sonodynamic action of Hypocrellin B induced mitochondrial damage, survival inhibition, and apoptosis of HepG2 cells. Additionally, other subcellular organelles such as endoplasmic reticulum, Golgi apparatus and lysosomes were also the targets of Hypocrellin B-mediated sonodynamic action as well as mitochondria. | | Photodiagnosis Photodyn Ther. 2012 Dec;9(4):337-43. | | Apoptosis of breast cancer cells induced by hypocrellin B under light-emitting diode irradiation.[Pubmed: 23200015] | Hypocrellin B was used in the present study to investigate apoptosis induction in breast cancer MDA-MB-231 cells.
METHODS AND RESULTS:
Photocytotoxicity was investigated 24h after photodynamic treatment of Hypocrellin B using MTT reduction assay and light microscopy. Hypocrellin B-induced photocytotoxicity in MDA-MB-231 cells exhibited a dose-dependent manner. The amount of MDA-MB-231 cells attached to the bottom of well decreased significantly after photodynamic treatment of Hypocrellin B. Flow cytometry showed that the early and late apoptotic rate of MDA-MB-231 cells increased remarkably up to 17.46% and 32.80%, respectively, after treatment of LED-activated Hypocrellin B. In addition, nuclear condensation, fragmentation and chromatin margination, and topical apoptotic body in the treated cells were observed by nuclear staining and TEM.
CONCLUSIONS:
Photodynamic action of Hypocrellin B irradiated by light-emitting diodes could significantly kill breast cancer cells and induce apoptotic cell death, which suggests LED-activated Hypocrellin B is a promising strategy for combating breast cancer. |
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