| Animal Research: |
| Int Angiol. 2008 Feb;27(1):81-5. | | Different flavonoids present in the micronized purified flavonoid fraction (Daflon 500 mg) contribute to its anti-hyperpermeability effect in the hamster cheek pouch microcirculation.[Pubmed: 18277344] | This study evaluated microcirculatory effects of the flavonoid substances that constitute the micronized purified flavonoid fraction (MPFF) (Daflon 500 mg) in comparison to diosmin.
METHODS AND RESULTS:
In groups of 3 male hamsters, oral treatment with MPFF or diosmin (15 min before anesthesia) did not alter blood pressure. At 10 or 30 mg/kg, both MPFF and diosmin significantly decreased the leaky sites caused by ischemia/reperfusion (I/R) (30 min) in the hamster cheek pouch; the effect was significantly higher with MPFF (39+/-1% and 52+/-1%, respectively) than diosmin (18+/-1% and 37+/-3%, respectively). Eight groups of 3 hamsters each were treated with the components of MPFF. Diosmetin only decreased the number leaky sites at 30 mg/kg (decrease: 15+/-2%). The decrement at 10 and 30 mg/kg averaged at: 17+/-3% and 44+/-1%, respectively, for hesperidin; 19+/-1% and 46+/-2%, respectively, for linarin; and 30+/-1% and 44+/-1%, respectively, for Isorhoifolin. Hesperidin, linarin, and Isorhoifolin each displayed an anti-leakage effect comparable to or greater than diosmin. MPFF decreases permeability more than any of its single constituents, suggesting that the flavonoids present in its formulation have a synergistic action.
CONCLUSIONS:
These results illustrate that MPFF is more potent than single diosmin in this model of hyperpermeability and that each of the flavonoid substances present in MPFF contribute to its action. | | Biomedicine & Preventive Nutrition,2011,1(4): 268-272. | | Flavonoid rich fraction of Pilea microphylla (L.) attenuates metabolic abnormalities and improves pancreatic function in C57BL/KsJ-db/db mice[Reference: WebLink] | Pilea microphylla Linn. (Urticaceae) and related genera are used in Jamaican and Chinese traditional medicine for the treatment of diabetes. This study was aimed at evaluating the antidiabetic potential of flavonoid rich fraction of Pilea microphylla (PM1) and its possible mechanism of action in C57BL/KsJ-db/db mice.
METHODS AND RESULTS:
These diabetic mice were treated with PM1 (100 mg/kg, i.p.) for 21 days and were evaluated for parameters such as food efficiency ratio, oral glucose tolerance test, inhibition of plasma dipeptidyl peptidase IV (DPP-IV), metabolic markers, pancreas histology and hematological profile. PM1 significantly (P < 0.05) reduced body weight, food efficiency ratio, plasma glucose, triglycerides and inhibited plasma DPP-IV, while increasing insulin levels in C57BL/KsJ-db/db mice. PM1 also improved oral glucose tolerance significantly (P < 0.05) with mean percentage reduction of 31.2 in glucose excursion (AUC0−120 min) and preserved islet architecture of pancreas without showing any detectable hematological toxicity at administered dose. The presence of flavonoids namely quercetin (reported DPP-IV inhibitor), rutin, chlorogenic acid (reported lipid lowering property) along with others (luteolin-7-O-glucoside, apigenin-7-O-glucoside, Isorhoifolin) in PM1 reversed the disturbed metabolic milieu in C57BL/KsJ-db/db mice.
CONCLUSIONS:
The overall antidiabetic effect could be the result of the combination of several constituents acting in concert, in a holistic manner, thereby restoring the homeostasis in energy consumption and utilization. |
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)