Science | Nature | Cell | View More
Natural Products
SB203580
SB203580
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name SB203580
Price:
CAS No.: 152121-47-6
Catalog No.: CFN60013
Molecular Formula: C21H16FN3OS
Molecular Weight: 377.43 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Powder
Source:
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS
Similar structural: Comparison
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • The Australian National University (Australia)
  • University of Stirling (United Kingdom)
  • Srinakharinwirot University (Thailand)
  • Mahidol University (Thailand)
  • The University of Newcastle (Australia)
  • CSIRO - Agriculture Flagship (Australia)
  • Universidad Veracuzana (Mexico)
  • Cancer Research Initatives Foun... (Malaysia)
  • Tokyo Woman's Christian University (Japan)
  • Charles Sturt University (Denmark)
  • Massachusetts General Hospital (USA)
  • More...
Package
Featured Products
2,4-Dihydroxybenzoic acid

Catalog No: CFN70215
CAS No: 89-86-1
Price: $30/20mg
Testosterone

Catalog No: CFN90034
CAS No: 58-22-0
Price: $40/20mg
Corylin

Catalog No: CFN98902
CAS No: 53947-92-5
Price: $90/20mg
Isovalerylshikonin

Catalog No: CFN95222
CAS No: 52387-14-1
Price: $318/10mg
Ginsenoside Rk3

Catalog No: CFN92593
CAS No: 364779-15-7
Price: $180/10mg
Strychnine

Catalog No: CFN98130
CAS No: 57-24-9
Price: $60/20mg
Taurocholic acid

Catalog No: CFN96646
CAS No: 81-24-3
Price: $ / mg
Vicenin -2

Catalog No: CFN92031
CAS No: 23666-13-9
Price: $138/20mg
Ganoderenic acid D

Catalog No: CFN90300
CAS No: 100665-43-8
Price: $368/20mg
Pinoresinol diglucoside

Catalog No: CFN99994
CAS No: 63902-38-5
Price: $100/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: SB203580 (RWJ 64809, PB 203580) is a p38 MAPK inhibitor with IC50 of 0.3-0.5 μM in THP-1 cells, 10-fold less sensitive to SAPK3(106T) and SAPK4(106T) and blocks PKB phosphorylation with IC50 of 3-5 μM. SB203580 induces mitophagy and autophagy.
Targets: p38MAPK | SAPK3 | PKB | Autophagy
SB203580 Description
Source:
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
3-Methoxyshancigusin I

Catalog No: CFN95062
CAS No: N/A
Price: $413/5mg
New compound 6

Catalog No: CFN95259
CAS No: N/A
Price: $413/5mg
Nortrachelogenin-5'-C-beta-glucosi...

Catalog No: CFN95251
CAS No: 858127-39-6
Price: $318/10mg
2''-O-Galloylquercitrin

Catalog No: CFN95041
CAS No: 80229-08-9
Price: $418/5mg
Naringin 4'-glucoside

Catalog No: CFN95014
CAS No: 17257-21-5
Price: $338/5mg
Baishouwubenzophenone

Catalog No: CFN95473
CAS No: 115834-34-9
Price: $318/5mg
trans-Psoralenoside

Catalog No: CFN95349
CAS No: N/A
Price: $318/5mg
Perlolyrine

Catalog No: CFN95291
CAS No: 29700-20-7
Price: $413/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.6495 mL 13.2475 mL 26.495 mL 52.99 mL 66.2374 mL
5 mM 0.5299 mL 2.6495 mL 5.299 mL 10.598 mL 13.2475 mL
10 mM 0.2649 mL 1.3247 mL 2.6495 mL 5.299 mL 6.6237 mL
50 mM 0.053 mL 0.2649 mL 0.5299 mL 1.0598 mL 1.3247 mL
100 mM 0.0265 mL 0.1325 mL 0.2649 mL 0.5299 mL 0.6624 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Br J Pharmacol,2000 Sep;131(1):99-107.
The p38 MAP kinase inhibitor SB203580 enhances nuclear factor-kappa B transcriptional activity by a non-specific effect upon the ERK pathway.[Pubmed: 10960075]

METHODS AND RESULTS:
In the present study we investigated a possible role for the p38 mitogen-activated protein (MAP) kinase pathway in mediating nuclear factor-kappa B (NF-kappaB) transcriptional activity in the erythroleukaemic cell line TF-1. TF-1 cells stimulated with the phosphatase inhibitor okadaic acid (OA) demonstrated enhanced NF-kappaB and GAL4p65-regulated transcriptional activity which was associated with elevated p38 phosphorylation. However, pretreatment with the p38 MAPK specific inhibitor SB203580 (1 microM) or overexpression of kinase-deficient mutants of MKK3 or MKK6 did not affect OA-enhanced NF-kappaB transcriptional potency, as determined in transient transfection assays. In fact, 5 and 10 microM SB203580 enhanced rather than inhibited NF-kappaB-mediated promoter activity by 2 fold, which was independent of phosphorylation of the p65 subunit. The SB203580-mediated increase in NF-kappaB transcriptional activity was associated with enhanced phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 kinase. Overexpression of kinase-deficient mutants belonging to the ERK1/2, JNK, and p38 pathways showed that only dominant-negative Raf-1 abrogated SB203580-enhanced NF-kappaB activity. This would implicate the involvement of the ERK1/2 pathway in the enhancing effects of SB203580 on NF-kappaB-mediated gene transcription.
CONCLUSIONS:
This study demonstrates that the p38 MAP kinase pathway is not involved in the OA-induced activation of NF-kappaB. SB203580 at higher concentrations activates the ERK pathway, which subsequently enhances NF-kappaB transcriptional activity.
Cell Research:
J Biol Chem,2000 Mar 10;275(10):7395-402.
The pyridinyl imidazole inhibitor SB203580 blocks phosphoinositide-dependent protein kinase activity, protein kinase B phosphorylation, and retinoblastoma hyperphosphorylation in interleukin-2-stimulated T cells independently of p38 mitogen-activated protein kinase.[Pubmed: 10702313]
Cell lines: CT6 cell , BA/F3 F7 cell
Concentrations:  0–30 μM
Incubation Time:  1 hour
Method:
CT6 cell and BA/F3 F7 cell are rested by washing three times in RPMI and culturing overnight in RPMI, 5% fetal calf serum in the absence of growth factor, antibiotics, or β-mercaptoethanol supplements. 2–5 × 106 rested CT6 cells are resuspended in 2 mL of RPMI, 5% fetal calf serum and preincubated with SB203580 or vehicle control as indicated in figure legends. Cells are then stimulated with 20 ng/ml recombinant human IL-2 for 5 minutes at 37  °C and pelleted in a minifuge for 30 seconds, medium is aspirated, and the pellet is lysed in the appropriate buffer. BA/F3 cells stably expressing deletion mutants of IL-2 receptor β chain are maintained in glutamine containing RPMI further supplemented with 5% fetal calf serum and 0.2 μg/mL G418. The cells are then washed extensively, rested overnight, and washed again before activating with IL-2; such cell preparations are >90% T cells. Cellular proliferation assays are performed by measurement of [3H]thymidine incorporation.
Animal Research:
Int Immunopharmacol,2011 Sep;11(9):1319-26.
The selective p38 mitogen-activated protein kinase inhibitor, SB203580, improves renal disease in MRL/lpr mouse model of systemic lupus.[Pubmed: 21549858]
Animal Models: Systemic lupus erythematosus (SLE) are established in female MRL/lpr mice and female C57BL/6 mice
Dosages:0.1 M/day
Administration: Orally administered
2,4-Dihydroxybenzoic acid

Catalog No: CFN70215
CAS No: 89-86-1
Price: $30/20mg
Testosterone

Catalog No: CFN90034
CAS No: 58-22-0
Price: $40/20mg
Corylin

Catalog No: CFN98902
CAS No: 53947-92-5
Price: $90/20mg
Isovalerylshikonin

Catalog No: CFN95222
CAS No: 52387-14-1
Price: $318/10mg
Ginsenoside Rk3

Catalog No: CFN92593
CAS No: 364779-15-7
Price: $180/10mg
Strychnine

Catalog No: CFN98130
CAS No: 57-24-9
Price: $60/20mg
Taurocholic acid

Catalog No: CFN96646
CAS No: 81-24-3
Price: $ / mg
Vicenin -2

Catalog No: CFN92031
CAS No: 23666-13-9
Price: $138/20mg
Ganoderenic acid D

Catalog No: CFN90300
CAS No: 100665-43-8
Price: $368/20mg
Pinoresinol diglucoside

Catalog No: CFN99994
CAS No: 63902-38-5
Price: $100/20mg
Tags: buy SB203580 | SB203580 supplier | purchase SB203580 | SB203580 cost | SB203580 manufacturer | order SB203580 | SB203580 distributor