| In vitro: |
| Asian Pac J Trop Med. 2014 Jan;7(1):9-15. | | Antioxidant, lipid peroxidation inhibition and free radical scavenging efficacy of a diterpenoid compound sugiol isolated from Metasequoia glyptostroboides.[Pubmed: 24418075 ] | To investigate the antioxidant efficacy of a biologically active diterpenoid compound Sugiol isolated from Metasequoia glyptostroboides (M. glyptostroboides) in various antioxidant models.
METHODS AND RESULTS:
An abietane type diterpenoid Sugiol, isolated from ethyl acetate extract of M. glyptostroboides cones, was analyzed for its antioxidant efficacy as reducing power ability and lipid peroxidation inhibition as well as its ability to scavenge free radicals such as 1,1-diphenyl-2-picryl hydrazyl, nitric oxide, superoxide and hydroxyl radicals. The Sugiol showed significant and concentration-dependent antioxidant and free radical scavenging activities. Consequently, the Sugiol exerted lipid peroxidation inhibitory effect by 76.5% as compared to α-tocopherol (80.13%) and butylated hydroxyanisole (76.59%). In addition, the Sugiol had significant scavenging activities of 1,1-diphenyl-2-picryl hydrazyl, nitric oxide, superoxide and hydroxyl free radicals in a concentration-dependent manner by 78.83%, 72.42%, 72.99% and 85.04%, when compared to the standard compound ascorbic acid (81.69%, 74.62%, 73.00% and 73.79%) and α-tocopherol/butylated hydroxyanisole (84.09%, 78.61%, 74.45% and 70.02%), respectively.
CONCLUSIONS:
These findings justify the biological and traditional uses of M. glyptostroboides or its secondary metabolites as confirmed by its promising antioxidant efficacy. | | Planta Med. 2005 Apr;71(4):300-5. | | Anti-inflammatory activity of sugiol, a diterpene isolated from Calocedrus formosana bark.[Pubmed: 15856404] | Sugiol is a diterpene which was isolated and purified from alcohol extracts of the bark of Calocedrus formosana Florin (Cupressaceae).
Although Sugiol has low inhibitory activity against the DPPH radical, it could effectively reduce intracellular reactive oxygen species (ROS) production in lipopolysaccharide (LPS)-stimulated macrophages.
METHODS AND RESULTS:
The present study investigated the potential anti-inflammatory activity of Sugiol, and the relationship between signal transduction and inflammatory cytokines in vitro. A dose of 30 microM of Sugiol was effectively inhibitory for proIL-1beta, IL-1beta and TNF-alpha production, suggesting that Sugiol is bioactive against inflammation. Moreover, Sugiol reveals a capacity for suppressing the activation of mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK), and p38 mitogen-activated protein kinase (p38) activated by LPS-stimulation in J774A.1 murine macrophages. A low dosage of 10 microM of Sugiol completely inhibited ERK1/2 phosphorylation, while 30 microM effectively inhibited JNK1/2 and p38 phosphorylation in LPS-stimulated macrophages. In addition, Sugiol significantly inhibited LPS-induced ROS production.
CONCLUSIONS:
Our studies suggest that Sugiol's efficacy in inhibiting the inflammatory cytokines of IL-1beta and TNF-alpha could be attributed to a reduction of the ROS that leads to a decrease in the phosphorylation of MAPKs. |
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