| In vitro: |
| Food Chem Toxicol. 2012 Feb;50(2):409-14. | | In vitro evaluation of tectoridin, tectorigenin and tectorigenin sodium sulfonate on antioxidant properties.[Pubmed: 22079309 ] | Tectoridin (4',5,7-thrihydroxy-6-methoxyisoflavone-7-O-β-d-glucopyranoside) isolated from the flowers of Pueraria thunbergiana is reported to have less hepatoprotective, hypoglycemic, antiallergic and anaphylaxis inhibitory activity than its aglycone form tectorigenin.
METHODS AND RESULTS:
To obtain tectorigenin, tectoridin was hydrolyzed in the current study. However, practical limitations of tectorigenin do exist due to its poor water-solubility. To increase its water-solubility, tectorigenin was sulfonated with sulfuric acid (98wt.%) and mixed with saturated salt water to produce Tectorigenin sodium sulfonate. Tectoridin and the two transfer products were identified by UV, IR, HPLC-MS, (1)H NMR and (13)C NMR, and the solubility of Tectorigenin sodium sulfonate was increased about 9-fold than tectorigenin. Antioxidant experiments of tectoridin, tectorigenin and modified tectorigenin in vitro including reducing power, superoxide anion radical scavenging activity, hydroxyl radical scavenging activity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity and anti-lipid peroxidation were carried on comparing with ascorbic acid (Vc) or butylated hydroxytoluene (BHT).
CONCLUSIONS:
The results suggested that the antioxidant activity in all the experimental systems exhibited the same order as follows: Tectorigenin sodium sulfonate>tectorigenin>tectoridin. Due to the high water-solubility and good antioxidant properties with Tectorigenin sodium sulfonate, appropriate chemical modifications could greatly improve the biological activities of the naturally occurring products. |
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