Science | Nature | Cell | View More
Natural Products
Betaine
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Betaine
Price: $30 / 20mg
CAS No.: 107-43-7
Catalog No.: CFN99546
Molecular Formula: C5H11NO2
Molecular Weight: 117.15 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Powder
Source: The roots of Beta vulgaris.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $7.0 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Rio de Janeiro State University (Brazil)
  • University of South Australia (Australia)
  • Monash University Malaysia (Malaysia)
  • University of Otago (New Zealand)
  • University of Pretoria (South Africa)
  • Kyung Hee University (Korea)
  • Tohoku University (Japan)
  • Universitas Airlangga (Indonesia)
  • University of Parma (Italy)
  • University of Wuerzburg (Germany)
  • University of Virginia (USA)
  • More...
Package
Featured Products
Oxypeucedanin hydrate

Catalog No: CFN90557
CAS No: 2643-85-8
Price: $80/20mg
Corydaline

Catalog No: CFN90196
CAS No: 518-69-4
Price: $138/20mg
Didymin

Catalog No: CFN92363
CAS No: 14259-47-3
Price: $80/20mg
Cryptochlorogenic acid

Catalog No: CFN99117
CAS No: 905-99-7
Price: $90/20mg
Liquiritin apioside

Catalog No: CFN90707
CAS No: 74639-14-8
Price: $90/20mg
Pinoresinol 4-O-beta-D-glucopyrano...

Catalog No: CFN97180
CAS No: 69251-96-3
Price: $100/20mg
1-Acetyl-beta-carboline

Catalog No: CFN92122
CAS No: 50892-83-6
Price: $ / mg
Gypenoside L

Catalog No: CFN91854
CAS No: 94987-09-4
Price: $268/5mg
Chlorogenic acid

Catalog No: CFN99116
CAS No: 327-97-9
Price: $30/20mg
Campesterol

Catalog No: CFN92204
CAS No: 474-62-4
Price: $188/10mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Betaine protects cells from environmental stress and serves as a methyl donor in several biochemical pathways, it reduces cardiovascular disease risk and protects liver cells from alcoholic liver damage and nonalcoholic steatohepatitis. Betaine has anti-inflammation and tumor preventing capacity, it can inhibit inflammatory cytokines such TNF-α, IL-6, iNOS and COX-2 and decrease the incidence of tumor formation with downregulation of inflammation.
Targets: TNF-α | IL Receptor | NOS | COX | NF-kB
In vitro:
Biochem Biophys Res Commun. 2015 Jan 9;456(2):621-5.
Betaine is a positive regulator of mitochondrial respiration.[Pubmed: 25498545]
Betaine protects cells from environmental stress and serves as a methyl donor in several biochemical pathways. It reduces cardiovascular disease risk and protects liver cells from alcoholic liver damage and nonalcoholic steatohepatitis. Its pretreatment can rescue cells exposed to toxins such as rotenone, chloroform, and LiCl. Furthermore, it has been suggested that Betaine can suppress cancer cell growth in vivo and in vitro. Mitochondrial electron transport chain (ETC) complexes generate the mitochondrial membrane potential, which is essential to produce cellular energy, ATP. Reduced mitochondrial respiration and energy status have been found in many human pathological conditions including aging, cancer, and neurodegenerative disease.
METHODS AND RESULTS:
In this study we investigated whether Betaine directly targets mitochondria. We show that Betaine treatment leads to an upregulation of mitochondrial respiration and cytochrome c oxidase activity in H2.35 cells, the proposed rate limiting enzyme of ETC in vivo. Following treatment, the mitochondrial membrane potential was increased and cellular energy levels were elevated.
CONCLUSIONS:
We propose that the anti-proliferative effects of Betaine on cancer cells might be due to enhanced mitochondrial function contributing to a reversal of the Warburg effect.
Int J Oncol. 2014 Sep;45(3):1250-6.
Anti-inflammatory effects of betaine on AOM/DSS‑induced colon tumorigenesis in ICR male mice.[Pubmed: 24969167]
Betaine is an important human nutrient obtained from various foods and studies in animals and humans have provided results suggesting their pathogenesis of various chronic diseases and points to a role in risk assessment and disease prevention. However, the molecular mechanisms of its activity remain poorly understood and warrant further investigation.
METHODS AND RESULTS:
This study was performed to investigate the anti-inflammation and tumor preventing capacity of Betaine on colitis-associated cancer in mice. In in vivo experiments, we induced colon tumors in mice by azoxymethane (AOM) and dextran sulfate sodium (DSS) and evaluated the effects of Betaine on tumor growth. Administration with Betaine significantly decreased the incidence of tumor formation with downregulation of inflammation. Treatment with Betaine inhibited ROS generation and GSSG concentration in colonic mucosa. Based on the qPCR data, administration of Betaine inhibited inflammatory cytokines such TNF-α, IL-6, iNOS and COX-2. In in vitro experiments, LPS-induced NF-κB and inflammatory-related cytokines were inhibited by Betaine treatment in RAW 264.7 murine macrophage cells.
CONCLUSIONS:
Our findings suggest that Betaine is one of the candidates for the prevention of inflammation-associated colon carcinogenesis.
In vivo:
Br J Pharmacol. 2014 Sep;171(17):4073-86.
Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease.[Pubmed: 24819676]
Overactive lipolysis in adipose tissue contributes to the pathogenesis of alcoholic liver disease (ALD); however, the mechanisms involved have not been elucidated. We previously reported that chronic alcohol consumption produces a hypomethylation state in adipose tissue. In this study we investigated the role of hypomethylation in adipose tissue in alcohol-induced lipolysis and whether its correction contributes to the well-established hepatoprotective effect of Betaine in ALD.
METHODS AND RESULTS:
Male C57BL/6 mice were divided into four groups and started on one of four treatments for 5 weeks: isocaloric pair-fed (PF), alcohol-fed (AF), PF supplemented with Betaine (BT/AF) and AF supplemented with Betaine (BT/AF). Betaine, 0.5% (w v(-1) ), was added to the liquid diet. Both primary adipocytes and mature 3T3-L1 adipocytes were exposed to demethylation reagents and their lipolytic responses determined. Betaine alleviated alcohol-induced pathological changes in the liver and rectified the impaired methylation status in adipose tissue, concomitant with attenuating lipolysis. In adipocytes, inducing hypomethylation activated lipolysis through a mechanism involving suppression of protein phosphatase 2A (PP2A), due to hypomethylation of its catalytic subunit, leading to increased activation of hormone-sensitive lipase (HSL). In line with in vitro observations, reduced PP2A catalytic subunit methylation and activity, and enhanced HSL activation, were observed in adipose tissue of alcohol-fed mice. Betaine attenuated this alcohol-induced PP2A suppression and HSL activation.
CONCLUSIONS:
In adipose tissue, a hypomethylation state contributes to its alcohol-induced dysfunction and an improvement in its function may contribute to the hepatoprotective effects of Betaine in ALD.
Anticancer Res. 2015 Mar;35(3):1475-80.
Sulfobetaine (dimethylsulfoniopropionate) and glycine betaine show incompatible involvement in crucial Ehrlich ascites carcinoma in mice.[Pubmed: 25750300]

METHODS AND RESULTS:
The role of methylation reactions in cancer was examined using the methylating agents, sulfoBetaine [dimethylsulfonioproponate (DMSP)], and glycine Betaine (GB), in murine crucial Ehrlich ascites carcinoma (EAC) for up to 10 days. DMSP administration in EAC-bearing mice mitigated EAC, while GB administration clearly promoted EAC. However, the immune cell profiles did not differ largely between animals receiving DMSP and those receiving GB. Moreover, DMSP and GB had merely any effects on proliferation of EAC cells in vitro. Injection of DMSP into normal mice interestingly led to macrophage accumulation in the peritoneal cavity in a dose-dependent manner at early rearing.
CONCLUSIONS:
These results indicate that GB promoted EAC by the methylation of cancer promotor gene, whereas DMSP ameliorated EAC by the accumulation of activated macrophages with a rapid response and long life span during cancer progression.
Gastroenterology. 2003 May;124(5):1488-99.
Betaine decreases hyperhomocysteinemia, endoplasmic reticulum stress, and liver injury in alcohol-fed mice.[Pubmed: 12730887]
Alcohol-induced hyperhomocysteinemia has been reported in rats and humans. Hyperhomocysteinemia has been associated with endoplasmic reticulum (ER) stress leading to the activation of ER-dependent apoptosis or up-regulation of lipid synthesis. This novel ER stress mechanism of alcoholic liver injury was studied in the model of intragastric alcohol-fed mice.
METHODS AND RESULTS:
Effects of alcohol on gene expression were analyzed using cDNA microarrays, RT-PCR, and Western blots over a period of 6 weeks. Liver injury was examined by histologic staining and TUNEL. We observed fatty liver, increased hepatic necroinflammation and apoptosis, and hyperhomocysteinemia. Of 1176 toxicology-related genes, glucose-regulated proteins (GRP-78 and -94), growth arrest/DNA damage-inducible protein 153 (CHOP/GADD153), and caspase-12 indicative of an ER stress response were among the alcohol-responsive genes. Sterol regulatory element binding protein (SREBP-1) and HMG-CoA reductase also were enhanced with alcohol administration. RT-PCR and selective Western blots confirmed the alcohol-induced expression of ER stress-related apoptosis and lipid synthesis genes. Addition of 0.5% and maximal 1.5% Betaine to the alcohol diet reduced the elevated level of plasma homocysteine by 54% and more than 80% accompanied by a decrease in hepatic lipids and ER stress response. Betaine did not attenuate the ethanol-induced increase in tumor necrosis factor alpha or CD14 mRNA.
CONCLUSIONS:
The results strongly suggest that alcohol may modulate both apoptotic and fat synthetic gene expression through homocysteine-induced ER stress in chronic alcoholic mouse liver and that correction of hyperhomocysteinemia by Betaine or other approaches may be useful to prevent alcoholic liver disease.
Betaine Description
Source: The roots of Beta vulgaris.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Hydroxytyrosol 1-O-glucoside

Catalog No: CFN95367
CAS No: 76873-99-9
Price: $318/10mg
Peiioside B

Catalog No: CFN95336
CAS No: 1610618-91-1
Price: $318/5mg
Quercetin-3'-glucoside

Catalog No: CFN95271
CAS No: 19254-30-9
Price: $318/10mg
3-O-[5'''-O-feruloyl-beta-D-apiofu...

Catalog No: CFN95154
CAS No: 148210-00-8
Price: $413/5mg
New compound 15

Catalog No: CFN95413
CAS No: N/A
Price: $413/5mg
Orthosiphol A

Catalog No: CFN95442
CAS No: 142741-25-1
Price: $318/5mg
Mahuannin G

Catalog No: CFN95553
CAS No: N/A
Price: $318/5mg
Mirificin-4'-O-glucoside

Catalog No: CFN95091
CAS No: 168035-01-6
Price: $288/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 8.5361 mL 42.6803 mL 85.3606 mL 170.7213 mL 213.4016 mL
5 mM 1.7072 mL 8.5361 mL 17.0721 mL 34.1443 mL 42.6803 mL
10 mM 0.8536 mL 4.268 mL 8.5361 mL 17.0721 mL 21.3402 mL
50 mM 0.1707 mL 0.8536 mL 1.7072 mL 3.4144 mL 4.268 mL
100 mM 0.0854 mL 0.4268 mL 0.8536 mL 1.7072 mL 2.134 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Cell Research:
Exp Eye Res. 2013 Mar;108:33-41.
Betaine stabilizes cell volume and protects against apoptosis in human corneal epithelial cells under hyperosmotic stress.[Pubmed: 23246691 ]
Cell lines:Human corneal limbal epithelial (HCLE) cells
Concentrations: 5 or 10 mM
Incubation Time: 16 h
Method:
Human corneal limbal epithelial (HCLE) cells exposed to culture medium for 16 h at 300 mOsm (isotonic) or 500 mOsm (hyperosmotic) in the presence or absence of Betaine (5 or 10 mM) are evaluated for cell volume changes; cell viability; and apoptosis.
Animal Research:
J Neuroinflammation. 2011; 8: 153.
Effects of betaine on lipopolysaccharide-induced memory impairment in mice and the involvement of GABA transporter 2[Pubmed: 22053950]
Animal Models: Male ddY strain mice
Formulation: 0.9% saline
Dosages:0.081, 0.163, or 0.326 mmol/kg
Administration: s.c.
Oxypeucedanin hydrate

Catalog No: CFN90557
CAS No: 2643-85-8
Price: $80/20mg
Corydaline

Catalog No: CFN90196
CAS No: 518-69-4
Price: $138/20mg
Didymin

Catalog No: CFN92363
CAS No: 14259-47-3
Price: $80/20mg
Cryptochlorogenic acid

Catalog No: CFN99117
CAS No: 905-99-7
Price: $90/20mg
Liquiritin apioside

Catalog No: CFN90707
CAS No: 74639-14-8
Price: $90/20mg
Pinoresinol 4-O-beta-D-glucopyrano...

Catalog No: CFN97180
CAS No: 69251-96-3
Price: $100/20mg
1-Acetyl-beta-carboline

Catalog No: CFN92122
CAS No: 50892-83-6
Price: $ / mg
Gypenoside L

Catalog No: CFN91854
CAS No: 94987-09-4
Price: $268/5mg
Chlorogenic acid

Catalog No: CFN99116
CAS No: 327-97-9
Price: $30/20mg
Campesterol

Catalog No: CFN92204
CAS No: 474-62-4
Price: $188/10mg
Tags: buy Betaine | Betaine supplier | purchase Betaine | Betaine cost | Betaine manufacturer | order Betaine | Betaine distributor