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(-)-Bicuculline methochloride
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name (-)-Bicuculline methochloride
Price:
CAS No.: 53552-05-9
Catalog No.: CFN92860
Molecular Formula: C21H20NO6.Cl
Molecular Weight: 417.8 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Powder
Source: The tubers of Corydalis ambigua
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison (Web)  (SDF)
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Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
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Biological Activity
Description: (-)-Bicuculline methochloride is a GABAA-receptor antagonist. Exposure to 100 microM (-)-Bicuculline methochloride for 48 hr can result in prominent CA1 cell death.
Targets: GABA Receptor
In vitro:
Int J Neurosci. 2010 Dec;120(12):752-9.
Effect of low Mg2+ and bicuculline on cell survival in hippocampal slice cultures.[Pubmed: 20942591]
A reliable model system of epileptiform insult would facilitate investigation into the underlying biological mechanisms.
METHODS AND RESULTS:
Epileptiform insult was induced in hippocampal slice cultures by lowering extracellular Mg(2+), (+)-bicuculline, or (-)-Bicuculline methochloride, a stable salt form of bicuculline (both forms block GABA(A) receptors). Cell death was assessed by propidium iodide uptake. Low Mg(2+) or (+)-bicuculline did not produce cell death regardless of dose or incubation period. Exposure to 100 microM (-)-Bicuculline methochloride for 48 hr resulted in prominent CA1 cell death.
CONCLUSIONS:
These findings demonstrate that not all pro-epileptic drugs/ion changes used routinely for electrophysiological recording of seizure activity lead to cell death in hippocampal slice cultures and that treatment with bicuculline methochloride can be used as a reliable model for epileptiform insult.
(-)-Bicuculline methochloride Description
Source: The tubers of Corydalis ambigua
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Cell. 2018 Jan 11;172(1-2):249-261.e12.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.3935 mL 11.9674 mL 23.9349 mL 47.8698 mL 59.8372 mL
5 mM 0.4787 mL 2.3935 mL 4.787 mL 9.574 mL 11.9674 mL
10 mM 0.2393 mL 1.1967 mL 2.3935 mL 4.787 mL 5.9837 mL
50 mM 0.0479 mL 0.2393 mL 0.4787 mL 0.9574 mL 1.1967 mL
100 mM 0.0239 mL 0.1197 mL 0.2393 mL 0.4787 mL 0.5984 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
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