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Estriol 3-sulfate
Estriol 3-sulfate
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Product Name Estriol 3-sulfate
Price:
CAS No.: 481-95-8
Catalog No.: CFN90080
Molecular Formula: C18H24O6S
Molecular Weight: 368.5 g/mol
Purity: >=98%
Type of Compound: Steroids
Physical Desc.: Powder
Source: From human breast cyst fluid.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
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Biological Activity
Description: Estriol 3-sulfate acts through the hydrolyzed E3, E3-17-S is inactive because it is not hydrolyzed, so Estriol 3-sulfate can play an important role in the biological responses of this mammary cancer cell line.
Targets: Estrogen receptor | Progestogen receptor
In vivo:
Steroids. 1984 Mar;43(3):235-42.
Specific antisera for the radioimmunoassay of estriol 3-sulfate.[Pubmed: 6523541 ]

METHODS AND RESULTS:
Antisera were raised in male guinea pigs against 6-oxoEstriol 3-sulfate O-carboxymethyloxime-bovine serum albumin (BSA) conjugate. The antisera to this antigen exhibited high affinity (Ka=4.7 X 10(9)M-1) and excellent specificity for Estriol 3-sulfate, showing slight cross-reactions (less than 0.43%) with other estrogen sulfates, and no cross-reactivities with free estrogens and other steroids (less than 0.01%) except cholesterol sulfate (0.22%).
CONCLUSIONS:
A standard curve using [6, 7-3H]-Estriol 3-sulfate as the radioactive ligand showed high sensitivity in the range of 10-1000 pg Estriol 3-sulfate.
Steroids. 1998 Oct;63(10):516-22.
Bridge-heterologous chemiluminescence enzyme-linked immunosorbent assay of estriol 3-sulfate in pregnancy plasma.[Pubmed: 9800282]

METHODS AND RESULTS:
A facile and sensitive chemiluminescence enzyme-linked immunosorbent assay (ELISA) of Estriol 3-sulfate using a bridge-heterologous system was established. 6 alpha-HydroxyEstriol 3-sulfate 6-hemisuccinate was synthesized as a novel hapten. Antisera were raised in male guinea-pigs against 6 alpha-hydroxyEstriol 3-sulfate 6-hemisuccinate-bovine serum albumin (BSA) and 6-oxoEstriol 3-sulfate O-carboxymethyloxime-BSA conjugates. Both haptens were coupled to horseradish peroxidase as an enzyme-label reagent. For separation of free and Estriol 3-sulfate bound to the antibody, the crude globulin fractions of these antisera were immobilized to CNBr-activated Sepharose-4B. The enzyme activity was measured by chemiluminescent reaction using amino-butylethylisoluminol and hydrogen peroxide as a substrate. The immobilized antibody raised against 6 alpha-hydroxyEstriol 3-sulfate 6-hemisuccinate-BSA exhibited a high affinity and an excellent specificity for Estriol 3-sulfate. The two bridge-heterologous ELISAs were more sensitive than the homologous systems. The specificity and sensitivity (10 pg) of the bridge-heterologous chemiluminescence ELISAs was comparable to those of the radioimmunoassays (RIAs).
CONCLUSIONS:
Results obtained by the ELISA and the RIA in pregnancy plasmas, showed excellent correlation between ELISA and RIA (r = 0.96).
J Clin Endocrinol Metab. 1981 Oct;53(4):847-51.
On the occurrence and transport of estriol-3-sulfate in human breast cyst fluid: the metabolic disposition of blood estriol-3-sulfate in normal women.[Pubmed: 7287868]

METHODS AND RESULTS:
The high concentration of Estriol 3-sulfate (E3-3S) in human breast cyst fluid has been confirmed in 14 women with multiple cysts. The concentrations of Estriol 3-sulfate found in individual cysts drained within a short time span from the same patient were variable, the ratios ranging from unity to 40. After the iv administration of [14C]estriol or [3H]Estriol 3-sulfate, only minor accumulation of either isotope was detected in the cyst fluids aspirated 6.5-30 h later. Since surprisingly small amounts of isotopes were found in blood, the metabolism of [3H]Estriol 3-sulfate was studied in 2 normal women. The test compound was injected iv, and blood samples were taken at intervals up to 7.5 h. In addition, total urine was collected for 3 days. The blood clearance of [3H]Estriol 3-sulfate was rapid, with the half-life ranging from 15-30 min. However, Estriol 3-sulfate was only a minor component of the urine, indicating rapid tissue extraction and metabolism rather than renal excretion for the compound.
CONCLUSIONS:
The studies indicate that Estriol 3-sulfate of human breast cyst fluid does not equilibrate rapidly with other body pools and that its uptake, if any, from the blood would be against a gradient.
Estriol 3-sulfate Description
Source: From human breast cyst fluid.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
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PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.7137 mL 13.5685 mL 27.137 mL 54.2741 mL 67.8426 mL
5 mM 0.5427 mL 2.7137 mL 5.4274 mL 10.8548 mL 13.5685 mL
10 mM 0.2714 mL 1.3569 mL 2.7137 mL 5.4274 mL 6.7843 mL
50 mM 0.0543 mL 0.2714 mL 0.5427 mL 1.0855 mL 1.3569 mL
100 mM 0.0271 mL 0.1357 mL 0.2714 mL 0.5427 mL 0.6784 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Cell Research:
J Steroid Biochem. 1986 Jan;24(1):357-9.
Effect of estriol, estriol-3-sulfate and estriol-17-sulfate on progesterone and estrogen receptors of MCF-7 human breast cancer cells.[Pubmed: 3702418]

METHODS AND RESULTS:
The levels of progesterone receptors (PR [cytosol (Cy) and nuclear (N)] and estrogen receptors (ER) [cytosol and nuclear; occupied and unoccupied specific binding sites] were evaluated in the MCF-7 cancer cell line incubated with estriol (E3), Estriol 3-sulfate (E3-3-S) or estriol-17-sulfate (E3-17-S) for 7 days in culture. Cells were grown in MEM medium containing 2 mM glutamine, 10% v/v dialysed calf serum and penicillin-streptomycin (100 U/ml) in the absence (control) or in the presence of 5 X 10(-8) M E3, Estriol 3-sulfate or E3-17-S. The total PR (Cy + N) concentration which was 0.47 +/- 0.10 (SE) pmol/mg DNA in the non-treated cells, increased to 1.95 +/- 0.48 in the E3 and to 1.55 +/- 0.26 in the Estriol 3-sulfate treated cells. No effect (PR: 0.47 +/- 0.15 pmol/mg DNA) was observed with the E3-17-S treatment. Total ER (Cy + N, occupied + unoccupied binding sites) in pmol/mg DNA +/- SE, were as follows: control 0.79 +/- 0.17; + E3: 0.33 +/- 0.09; +E3-3-S: 0.90 +/- 0.18 and +E3-17-S: 1.82 +/- 0.58. The measurement by radioimmunoassay of unconjugated estriol in the culture medium indicated that after incubation with Estriol 3-sulfate, a fraction (0.5-1%) of the sulfate was hydrolyzed but no hydrolysis was observed in the incubations with E3-17-S.
CONCLUSIONS:
It is concluded that in the MCF-7 human mammary cancer cell line E3 and Estriol 3-sulfate stimulate PR very significantly, but it is suggested that Estriol 3-sulfate acts through the hydrolyzed E3. On the other hand, E3-17-S is inactive because it is not hydrolyzed. Consequently, Estriol 3-sulfate can play an important role in the biological responses of this mammary cancer cell line.
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