Science | Nature | Cell | View More
Natural Products
Micheliolide
Micheliolide
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Micheliolide
Price: $258 / 20mg
CAS No.: 68370-47-8
Catalog No.: CFN91035
Molecular Formula: C15H20O3
Molecular Weight: 248.3 g/mol
Purity: >=98%
Type of Compound: Sesquiterpenoids
Physical Desc.: Powder
Source: The herbs of Michelia compressa
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $40.8 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • University of Eastern Finland (Finland)
  • University of Toulouse (France)
  • Universidad Veracuzana (Mexico)
  • VIB Department of Plant Systems... (Belgium)
  • Heinrich-Heine-University Düss... (Germany)
  • National Cancer Institute (USA)
  • Universiti Putra Malaysia(UPM) (Malaysia)
  • Korea Intitute of Science and T... (Korea)
  • Utah State University (USA)
  • University of Helsinki (Finland)
  • Max-Planck-Insitut (Germany)
  • More...
Package
Featured Products
Cyanidin-3-O-galactoside chloride

Catalog No: CFN92040
CAS No: 27661-36-5
Price: $318/10mg
Tenuifolin

Catalog No: CFN98157
CAS No: 20183-47-5
Price: $100/20mg
Mangiferin

Catalog No: CFN98719
CAS No: 4773-96-0
Price: $40/20mg
Linoleic acid

Catalog No: CFN93168
CAS No: 60-33-3
Price: $30/20mg
Oxyresveratrol

Catalog No: CFN98368
CAS No: 29700-22-9
Price: $30/20mg
Beta-Sitosterol

Catalog No: CFN99916
CAS No: 83-46-5
Price: $40/20mg
Soyasapogenol D

Catalog No: CFN91069
CAS No: 65892-76-4
Price: $463/5mg
Eupatorin

Catalog No: CFN97418
CAS No: 855-96-9
Price: $158/10mg
Delta 5-avenasterol

Catalog No: CFN92239
CAS No: 18472-36-1
Price: $413/5mg
(+)-Gallocatechin

Catalog No: CFN99138
CAS No: 970-73-0
Price: $138/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Micheliolide has antineoplastic, anti-inflammatory and immunomodulatory effects, it inhibits various inflammatory response and may serve as a neuroprotective agent in neuroinflammation-related neurodegenerative disorders. Micheliolide has potential as a candidate drug for the treatment of diabetic nephropathy, it can effectively attenuate the high glucose-stimulated activation of NF-κB, the degradation of IκBα, and the expression of MCP-1, TGF-β1 and FN in rat mesangial cells (MCs). Micheliolide also ameliorates liver steatosis by upregulating PPAR-γ expression, thereby inhibiting NF-κB-mediated inflammation and activating AMPK/mTOR-dependent autophagy.
Targets: PPAR | p65 | NF-kB | IkB | AMPK | mTOR | COX | TNF-α | IL Receptor | NO | NOS | Akt | JNK | p38MAPK | ERK | HO-1 | Nrf2 | PI3K | ROS | TGF-β/Smad | IKK
In vitro:
PLoS One. 2017 Oct 17;12(10):e0186592.
Micheliolide suppresses LPS-induced neuroinflammatory responses.[Pubmed: 29040306 ]
Microglia-involved neuroinflammation is thought to promote brain damage in various neurodegenerative disorders. Thus, inhibition of microglial over-activation may have a therapeutic benefit for the treatment of neurodegenerative disorders. Micheliolide (MCL) is a sesquiterpene lactone which inhibits various inflammatory response. However, whether MCL can inhibit neuroinflammation caused by LPS-activated BV2 microglia has not yet been explored.
METHODS AND RESULTS:
In this study, we demonstrated that treatment of BV2 cells with MCL significantly repressed LPS-stimulated nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, as well as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO) induction. MCL also attenuated mRNA levels of multiple pro-inflammatory cytokines and mediators such as iNOS, COX-2, TNF-α, IL-6 and IL-1β. Mechanistic studies revealed that MCL suppressed LPS-stimulated the activation of IκBα/NF-κB pathway and Akt pathway. Moreover, MCL inhibited LPS-induced the activition of c-Jun N-terminal kinase (JNK), p38 MAPK kinase, and extracellular signal-regulated kinases 1/2 (ERK1/2). Meanwhile, MCL markedly promoted antioxidant protein heme oxygenase-1 (HO-1) expression by enhancing NF-E2-related factor 2 (Nrf2) activity.
CONCLUSIONS:
Together, our results imply that MCL may serve as a neuroprotective agent in neuroinflammation-related neurodegenerative disorders.
Molecules. 2013 Oct 21;18(10):13061-77.
Sesquiterpene lactones and their derivatives inhibit high glucose-induced NF-κB activation and MCP-1 and TGF-β1 expression in rat mesangial cells.[Pubmed: 24152676 ]
Diabetic nephropathy (DN) is one of the most common and serious chronic complications of diabetes mellitus, however, no efficient clinical drugs exist for the treatment of DN.
METHODS AND RESULTS:
We selected and synthesized several sesquiterpene lactones (SLs), and then used the MTT assay to detect rat mesangial cells (MCs) proliferation, ELISA to measure the expression level of monocyte chemoattractant protein-1 (MCP-1), transforming growth factor beta (TGF-β1) and fibronectin(FN), real-time fluorescent quantitative PCR analysis to measure the MCP-1 and TGF-β1 gene expression, western blot to detect the level of IκBα protein and EMSA to measure the activation of nuclear factor kappa B (NF-κB). We discovered that SLs, including parthenolide (PTL), Micheliolide (MCL), arglabin, and isoalantolactone (IAL), as well as several synthetic analogs of these molecules, could effectively attenuate the high glucose-stimulated activation of NF-κB, the degradation of IκBα, and the expression of MCP-1, TGF-β1 and FN in rat mesangial cells (MCs).
CONCLUSIONS:
These findings suggest that SLs and their derivatives have potential as candidate drugs for the treatment of DN.
In vivo:
Int Immunopharmacol. 2018 Jun;59:197-208.
Micheliolide alleviates hepatic steatosis in db/db mice by inhibiting inflammation and promoting autophagy via PPAR-γ-mediated NF-кB and AMPK/mTOR signaling.[Pubmed: 29656210]
The anti-inflammatory, immunomodulatory, and anticancer effects of Micheliolide (MCL) isolated from Michelia champaca were previously reported, but its role and underlying mechanisms in relieving liver steatosis remain unclear.
METHODS AND RESULTS:
Herein, we investigated the effects of MCL on hepatic steatosis using a db/db mouse model and lipid mixture (LM)-induced AML12 and LO2 cells. The body and liver weights, food consumption, lipid content and liver aminotransferase levels in serum, the lipid content and inflammatory cytokine levels in liver tissue, and the extent of hepatic steatosis in db/db mice were increased compared with those in db/m mice, and these increases were reversed by MCL treatment. Similarly, MCL also attenuated the inflammatory responses and lipid accumulation in LM-treated AML12 and L02 cells by upregulating PPAR-γ and decreasing p-IкBα and p-NF-κB/p65, thereby inhibiting the NF-κB pathway and reducing lipotoxicity. Furthermore, MCL administration increased LC3B, Atg7 and Beclin-1 expression and the LC3B-II/I ratio in db/db mouse livers and LM-treated AML12 and L02 cells, and these MCL-induced increases were mediated by the activation of PPAR-γ and p-AMPK and inhibition of p-mTOR and induce autophagy. These effects were blocked by PPAR-γ and AMPK inhibitors.
CONCLUSIONS:
Our findings suggest that MCL ameliorates liver steatosis by upregulating PPAR-γ expression, thereby inhibiting NF-κB-mediated inflammation and activating AMPK/mTOR-dependent autophagy.
Oncotarget. 2016 Oct 4;7(40):65012-65023.
Antineoplastic effects and mechanisms of micheliolide in acute myelogenous leukemia stem cells.[Pubmed: 27542251]
Leukemic stem cells (LSCs) greatly contribute to the initiation, relapse, and multidrug resistance of leukemia. Current therapies targeting the cell cycle and rapidly growing leukemic cells, including conventional chemotherapy, have little effect due to the self-renewal and differentiated malignant cells replenishment ability of LSCs despite their scarce supply in the bone marrow. Micheliolide (MCL) is a natural guaianolide sesquiterpene lactone (GSL) which was discovered in michelia compressa and michelia champaca plants, and has been shown to exert selective cytotoxic effects on CD34+CD38- LSCs.
METHODS AND RESULTS:
In this study, we demonstrate that DMAMCL significantly prolongs the lifespan of a mouse model of human acute myelogenous leukemia (AML). Mechanistic investigations further revealed that MCL exerted its cytotoxic effects via inhibition of NF-κB expression and activity, and by generating intracellular reactive oxygen species (ROS). These results provide valuable insight into the mechanisms underlying MCL-induced cytotoxicity of LSCs, and support further preclinical investigations of MCL-related therapies for the treatment of AML.
Micheliolide Description
Source: The herbs of Michelia compressa
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
12beta-Acetoxy-3beta-hydroxy-7,11,...

Catalog No: CFN95468
CAS No: 1085338-75-5
Price: $318/5mg
Pinocembrin 7-O-(4'',6''-hexahydro...

Catalog No: CFN95462
CAS No: 1825287-22-6
Price: $318/10mg
cis-Aegineoside

Catalog No: CFN95295
CAS No: N/A
Price: $413/5mg
3-O-methylellagic acid 4'-O-alpha-...

Catalog No: CFN95228
CAS No: 51768-39-9
Price: $413/5mg
Dehydrojuncusol

Catalog No: CFN95143
CAS No: 117824-04-1
Price: $318/5mg
Gambogoic acid A

Catalog No: CFN95439
CAS No: 887923-49-1
Price: $318/10mg
Ginsenoside MC

Catalog No: CFN95232
CAS No: 175484-06-7
Price: $318/10mg
2-(2,4-Dihydroxyphenyl)-5,6-methyl...

Catalog No: CFN95511
CAS No: 67121-26-0
Price: $318/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 4.0274 mL 20.1369 mL 40.2739 mL 80.5477 mL 100.6847 mL
5 mM 0.8055 mL 4.0274 mL 8.0548 mL 16.1095 mL 20.1369 mL
10 mM 0.4027 mL 2.0137 mL 4.0274 mL 8.0548 mL 10.0685 mL
50 mM 0.0805 mL 0.4027 mL 0.8055 mL 1.611 mL 2.0137 mL
100 mM 0.0403 mL 0.2014 mL 0.4027 mL 0.8055 mL 1.0068 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Mediators Inflamm. 2017;2017:2432904.
MCL Plays an Anti-Inflammatory Role in Mycobacterium tuberculosis-Induced Immune Response by Inhibiting NF-κB and NLRP3 Inflammasome Activation.[Pubmed: 28642632]
Mycobacterium tuberculosis (Mtb) remains a significant menace to global health as it induces granulomatous lung lesions and systemic inflammatory responses during active tuberculosis (TB). Micheliolide (MCL), a sesquiterpene lactone, was recently reported to have a function of relieving LPS-induced inflammatory response, but the regulative role of MCL on the immunopathology of TB still remains unknown.
METHODS AND RESULTS:
In this experiment, we examined the inhibitory effect of MCL on Mtb-induced inflammatory response in mouse macrophage-like cell line Raw264.7 by downregulating the activation of nuclear factor kappa B (NF-κB) and NLRP3 inflammasome. Evidences showed that MCL decreased the secretion of Mtb-induced inflammatory cytokines (IL-1β and TNF-α) in a dose-dependent manner. Meanwhile, MCL dramatically suppressed Mtb-induced activation of iNOS and COX2 as well as subsequent production of NO. Furthermore, MCL inhibited Mtb-induced phosphorylation of Akt (Ser 473) in Raw264.7.
CONCLUSIONS:
According to our results, MCL plays an important role in modulating Mtb-induced inflammatory response through PI3K/Akt/NF-κB pathway and subsequently downregulating the activation of NLRP3 inflammasome. Therefore, MCL may represent as a potential drug candidate in the adjuvant treatment of TB by regulating host immune response.
Structure Identification:
J Asian Nat Prod Res. 2014;16(9):922-9.
New cytotoxic sesquiterpene lactones from Anthemis scrobicularis.[Pubmed: 25227949]
Four new sesquiterpene lactones, 4α-hydroxy-guaia-10(14),11(13)-diene-12,6α-olide (1), 4α-hydroxy-9α-acetoxy-guaia-1(10),2-diene-12,6α-olide (4), 4α-hydroxy-9β-acetoxy-guaia-1(10),2-diene-12,6α-olide (5), and 1α,4α-dihydroxy-9α-acetoxy-guaia-10(14),2-diene-12,6α-olide (6), were isolated from the aerial parts of Anthemis scrobicularis.
METHODS AND RESULTS:
Their structures were elucidated on the basis of their IR, NMR, and MS spectroscopic data. In addition, two known sesquiterpene lactones Micheliolide (2) and achillin (3) were also isolated. The cytotoxicity of some of the isolated compounds was tested against HCT 116, HepG-2, and MCF-7 cell lines. Micheliolide and 4α-hydroxy-guaia-10(14),11(13)-diene-12,6α-olide showed pronounced inhibitory activity while 4α-hydroxy-9α-acetoxy-guaia-1(10),2-diene-12,6α-olide showed weak activity.
Cyanidin-3-O-galactoside chloride

Catalog No: CFN92040
CAS No: 27661-36-5
Price: $318/10mg
Tenuifolin

Catalog No: CFN98157
CAS No: 20183-47-5
Price: $100/20mg
Mangiferin

Catalog No: CFN98719
CAS No: 4773-96-0
Price: $40/20mg
Linoleic acid

Catalog No: CFN93168
CAS No: 60-33-3
Price: $30/20mg
Oxyresveratrol

Catalog No: CFN98368
CAS No: 29700-22-9
Price: $30/20mg
Beta-Sitosterol

Catalog No: CFN99916
CAS No: 83-46-5
Price: $40/20mg
Soyasapogenol D

Catalog No: CFN91069
CAS No: 65892-76-4
Price: $463/5mg
Eupatorin

Catalog No: CFN97418
CAS No: 855-96-9
Price: $158/10mg
Delta 5-avenasterol

Catalog No: CFN92239
CAS No: 18472-36-1
Price: $413/5mg
(+)-Gallocatechin

Catalog No: CFN99138
CAS No: 970-73-0
Price: $138/20mg
Tags: buy Micheliolide | Micheliolide supplier | purchase Micheliolide | Micheliolide cost | Micheliolide manufacturer | order Micheliolide | Micheliolide distributor