| In vitro: |
| Letters in Applied Microbiology, 1993, 17(1):29-32. | | Growth-Inhibition of Selected Food-Borne Bacteria by Tannic-Acid, Propyl Gallate and Related-Compounds[Reference: WebLink] |
METHODS AND RESULTS:
Tannic acid, Propyl gallate, gallic acid and ellagic acid were tested for their inhibitory effects on selected food-borne bacteria by the well assay technique.
CONCLUSIONS:
Tannic acid and Propyl gallate were inhibitory whereas gallic acid and ellagic acid were not. | | J Agric Food Chem. 2003 Feb 12;51(4):1077-80. | | Sequestering ability of butylated hydroxytoluene, propyl gallate, resveratrol, and vitamins C and E against ABTS, DPPH, and hydroxyl free radicals in chemical and biological systems.[Pubmed: 12568575 ] | The antioxidant capacity of butylated hydroxytoluene (BHT; 2,6-di-tert-butyl-p-cresol), Propyl gallate (3,4,5-trihydroxybenzoic acid n-propyl ester), resveratrol (trans-3,4',5-trihydroxystilbene), and vitamins C (l-ascorbic acid) and E [(+)-alpha-tocopherol] was studied in chemical and biological systems.
METHODS AND RESULTS:
The chemical assays evaluated the capacity of these antioxidants to sequester 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.) and 1,1 diphenyl-2-picrylhydrazyl (DPPH.). A new colorimetric method to determine hydroxyl radical scavenging is also described. The biological tests use the eucaryotic cells of Saccharomyces cerevisiae treated with the antioxidants in the presence of the stressing agents apomorphine, hydrogen peroxide, and paraquat dichloride (methylviologen; 1,1'-dimethyl-4,4'-bipyridinium dichloride).
The results in chemical systems showed that all of the antioxidants were able to significantly inhibit the oxidation of beta-carotene by hydroxyl free radicals.
The assays in yeast showed that the antioxidant activity of the tested compounds depended on the stressing agent used and the mechanism of action of the antioxidant. | | Biochemical Pharmacology, 1994, 48(2):419-422. | | Propyl gallate as a hepatoprotector in vitro and in vivo.[Pubmed: 8053938] |
Recently, there has been renewed interest in Propyl gallate, a preservative in foods and fuels. This compound, which exhibits antimicrobial activity, has been found to be toxicologically safe after almost 30 years of evaluation.
METHODS AND RESULTS:
In the present study, we examined whether Propyl gallate is a hepatoprotective antioxidant, and investigated some of its bases of action vis-à-vis Trolox, a vitamin E analogue. In isolated rat hepatocytes, Propyl gallate prolonged substantially cell survival against oxyradicals generated with xanthine oxidase-hypoxanthine. The protection was dose dependent and excelled that of Trolox, mannitol, or ascorbate, each at or near its optimum level in the same system. In rats undergoing an 80-min partial hepatic ischemia, infusion of Propyl gallate at 20 mumol/kg body weight just before a 24-hr reperfusion salvaged the organ by 80.0 +/- 11.5%, an extent comparable to that with Trolox. Mechanistically, we found that Propyl gallate (a) protected hepatocytes against the cascade of oxyradicals produced by xanthine oxidase-hypoxanthine; (b) protected hepatocytes against superoxide radicals generated specifically by menadione; (c) protected the functionally important hepatic vascular endothelial cells more effectively than Trolox against xanthine oxidase-hypoxanthine, and (d) approximately halved the amount of lipid conjugated dienes (a more specific marker of oxyradical damage than malondialdehyde) formed in tissues after oxidant damage.
CONCLUSIONS:
Therefore, there are fundamental reasons why Propyl gallate is an effective antioxidant-based hepatoprotector, both in vitro and in vivo. |
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